Effect of LINC00852 Regulating the miR-671-5p/MYH9 Signaling Axis on Proliferation Invasion and Migration of Non-Small Cell Lung Cancer Cells
Objective:To investigate the effects of long non-coding RNA 00852(LINC00852)on the proliferation,invasion,and migration of non-small cell lung cancer(NSCLC)cells by regulating the microR-NA-671-5p(miR-671-5p)/myosin heavy chain 9(MYH9)signaling axis.Methods:The expression levels of LINC00852,miR-671-5p,and MYH9 mRNA were detected in NSCLC tissues and adjacent normal tissues using qRT-PCR.A549 cells were randomly divided into several groups:Control group,sh-NC group,sh-LINC00852 group,sh-LINC00852+anti-NC group,and sh-LINC00852+anti-miR-671-5p group.The ex-pression levels of LINC00852,miR-671-5p,and MYH9 mRNA were detected.Cell proliferation,invasion,and migration were assessed by colony formation assay,Transwell assay,and wound healing assay,respective-ly.Western blot was used to detect the protein expression of PCNA,MYH9,and MMP-9.Dual-luciferase reporter assay was performed to verify the interaction between LINC00852 and miR-671-5p,and between miR-671-5p and MYH9.Results:The expression levels of LINC00852 and MYH9 mRNA were significantly higher in NSCLC tissues compared with adjacent normal tissues,while the expression of miR-671-5p was sig-nificantly lower.Knockdown of LINC00852 inhibited the proliferation,invasion,and migration of A549 cells,and downregulated the expression of MYH9,PCNA,and MMP-9,while upregulating the expression of miR-671-5p.Dual-luciferase reporter assay confirmed that LINC00852 could directly target miR-671-5p,and miR-671-5p could directly target MYH9.Conclusion:LINC00852 inhibits the proliferation,invasion,and migration of NSCLC cells by regulating the miR-671-5p/MYH9 signaling axis.
Long non-coding RNA 00852microRNA-671-5pMyosin heavy chain 9Non-small cell lung cancerProliferationInvasionMigration
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