Effect of Exosomal Transport of circ_001895 from Type 2 Diabetes Mellitus on Endothelial Progenitor Cells under High Glucose Stress
Objective:To investigate the effects of exosomal circ_001895 transportation on endothelial progenitor cells under high glucose stress derived from patients with type 2 diabetes(T2DM).Methods:Ser-um samples were obtained from T2DM patients and divided into serum group and EXO group.The expression levels of circ_001895 in serum and EXO were detected by using RT-qPCR technology.EXOs derived from T2DM patients'serum with significantly elevated circ_001895 expression were named EXO-circ.Endothelial progenitor cells provided by Wuhan Procell were cultured and divided into the following three groups.Control Group:Cultured conventionally for 24 hours without additional treatment.High Glucose(HG)Group:Trea-ted with 30 mmoL/L glucose for 24 hours.HG+EXO-circ Group:Treated with 30 mmoL/L glucose combined with 100μg/mL EXO-circ for 24 hours.The expression of circ_001895 in each group of cells was detected u-sing RT-qPCR technology.Cell proliferation vitality was detected using the CCK-8 method.Cell apoptosis was detected using flow cytometry.The expression of angiogenic markers E-cadherin,N-cadherin,and Vim-entin was detected using Western blot technology.Cell migration capacity was detected using the Transwell cell migration assay.Results:Compared to the serum group,the relative expression level of circ_001895 in the EXO group was significantly upregulated(P<0.05).Compared to the control group,the relative expres-sion levels of circ_001895 and E-cadherin and the cell apoptosis rate in the HG group were significantly up-regulated(all P<0.05),while cell proliferation vitality,migration capacity,and the relative expression levels of N-cadherin and Vimentin were significantly downregulated(all P<0.05).Compared to the HG group,the relative expression levels of circ_001895 and E-cadherin and the cell apoptosis rate in the HG+EXO-circ group were significantly upregulated(all P<0.05),while cell proliferation vitality,migration capacity,and the relative expression levels of N-cadherin and Vimentin were significantly downregulated(all P<0.05).Conclusion:Transportation of circ_001895 in exosomes from T2DM inhibits proliferation,migration,and vas-cular generation of endothelial progenitor cells under high glucose stress,while promoting apoptosis.
Type 2 diabetesExosomescirc_001895Endothelial progenitor cellsMigra-tionVascular generation
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维普
