Effect of Curcumin on Lung Injury in Mice with ARDS via Regulation of the MAPK/NF-κB Signaling Pathway
Objective:To investigate the protective effects of curcumin on acute respiratory distress syn-drome(ARDS)in mice by regulating the MAPK/NF-κB signaling pathway.Methods:BALB/C mice were randomly divided into five groups:control group,model group,curcumin group(200mg/kg),anisomycin group(MAPK activator,20mg/kg),and curcumin(200mg/kg)+anisomycin(20mg/kg)group,with 12 mice per group.ARDS models were established in the model and drug intervention groups via intratracheal in-stillation of lipopolysaccharide(LPS),while the control group received an equal volume of normal saline.Af-ter 3 days of intervention,the following were measured:lung function indices-forced vital capacity(FVC),peak expiratory flow(PEF),and oxygenation index(OI);inflammatory cell count in bronchoalveolar lavage fluid(BALF);histopathological changes and lung injury scores in lung tissues via HE staining;levels of TNF-α,IL-1β,IL-10,and IL-13 in BALF and serum using ELISA;and MAPK/NF-κB pathway protein ex-pression in lung tissues via Western blotting.Results:Compared with the control group,the model group showed significantly increased inflammatory cell count,lung injury score,TNF-α and IL-1β levels,and p-p38 MAPK/p38 MAPK and p-NF-κB p65/NF-κB p65 ratios(P<0.05),while FVC,PEF,OI,IL-10,and IL-13 levels were significantly decreased(P<0.05).Compared with the model group,the curcumin group had reduced inflammatory cell count,lung injury score,TNF-α and IL-1β levels,and p-p38 MAPK/p38 MAPK and p-NF-κB p65/NF-κB p65 ratios(P<0.05),along with increased FVC,PEF,OI,IL-10,and IL-13 levels(P<0.05).The anisomycin group,compared to the model group,exhibited further increa-ses in inflammatory markers and reductions in lung function indices.Co-administration of curcumin and aniso-mycin negated the protective effects observed in the curcumin group alone.Conclusion:Curcumin inhibits the activation of the MAPK/NF-κB signaling pathway,reducing inflammation,mitigating lung tissue damage,and improving lung function in ARDS mice,thereby exerting a significant protective effect on the lungs.
万方数据
维普
