Exogenous Nerve Growth Factor Activates the Wnt3α/β-Catenin Signaling Pathway to Promote Fracture Healing
Objective:To analyze the effect of exogenous nerve growth factor on the activation of Wnt3α/β-catenin signaling pathway in promoting fracture healing.Methods:Select 40 SPF grade SD rats,10 rats as the control group,and 30 rats to construct a tibial fracture model.A total of 27 rats were successfully modeled and divided into a model group of 9 rats,a low-dose group of 9 rats,and a high-dose group of 9 rats.Ob-serve the pathological histology,bone calcium and phosphorus levels,hemorheological indicators,BMD,cal-lus diameter,angiogenesis related factors,inflammatory factor levels,mRNA expression levels of key genes Wn3a,β-catenin,and GSK-3β in the Wnt3 α/β-catenin pathway,and expression levels of key proteins Wnt3a,β-catenin,and GSK-3β in the Wnt3 α/β-catenin pathway in each group of rats.Results:Com-pared with the control group,the other three groups showed a decrease in bone calcium,bone phosphorus,BMD,angiogenesis related factors,Wnt3α,β-catenin related mRNA expression,Wnt3α,β-catenin expres-sion,and an increase in whole blood viscosity,plasma viscosity,inflammatory factor levels,GSK-3β related mRNA expression,and GSK-3β expression.Compared with the model group,the low and high dose groups showed an increase in bone calcium,bone phosphorus,BMD,callus diameter,angiogenesis related factors,Wnt3 α,β-catenin related mRNA expression,Wnt3α,β-catenin expression,and a decrease in whole blood viscosity,plasma viscosity,inflammatory factor levels,GSK-3 β related mRNA expression,and GSK-3β expression;And the high-dose group showed the highest levels of bone calcium,bone phosphorus,BMD,callus diameter,angiogenesis related factors,Wnt3α,β-catenin related mRNA expression,Wnt3α,β-cate-nin expression,and the lowest levels of whole blood viscosity,plasma viscosity,inflammatory factor levels,GSK-3β-related mRNA expression,and GSK-3β expression(P<0.05).Conclusion:The intervention of exogenous nerve growth factor on tibial bone can promote the healing of fracture in rats,the mechanism of which may be related to the activation of Wnt3α/β-catenin signaling pathway,and has a good effect.
万方数据
维普
