摘要
目的:观察系统性红斑狼疮(SLE)患儿血清白介素-35(IL-35)、C-C 基序趋化因子配体19(CCL19)变化情况,并分析其与患儿免疫学指标的关联性.方法:纳入 2022 年 1 月至 2024 年 3 月本院收治的SLE患儿97 例,并同期选取97 例健康儿童进行对照研究,观察患儿血清 IL-35、CCL19 水平变化,并分析其与免疫学指标(包括抗ds-DNA抗体、免疫球蛋白(Ig)及补体C3、C4)的相关性.结果:与健康儿童相比,SLE 患儿血清 IL-35 水平明显较低,CCL19 水平明显较高,差异均有统计学意义(P<0.05).与非活动期SLE患儿相比,活动期 SLE 患儿血清 IL-35、C3、C4 水平明显较低,CCL19、抗 ds-DNA抗体、IgM、IgG、IgA水平明显较高,差异均有统计学意义(P<0.05).Pearson 相关性分析显示:SLE患儿血清IL-35 水平与抗ds-DNA抗体、IgM、IgG、IgA 水平成负相关性,与 C3、C4 水平成正相关,差异均有统计学意义(r=-0.314、-0.203、-0.301、-0.201、0.318、0.383,P<0.05);SLE 患儿血清 CCL19 水平与抗ds-DNA抗体、IgM、IgG、IgA水平成正相关性,与C3、C4 水平成负相关差异均有统计学意义(r=0.272、0.209、0.362、0.202、-0.219、-0.391,P<0.05).线性回归显示:SLE患儿抗ds-DNA抗体与IL-35 水平存在负向影响关系,差异有统计学意义(P<0.05),C3 则与IL-35 水平存在正向影响关系,差异有统计学意义(P<0.05);SLE 患儿 IgG 与 CCL19 水平存在正向影响关系,差异有统计学意义(P<0.05).结论:SLE患儿血清IL-35、CCL19 水平异常变化,与抗ds-DNA抗体、IgM、IgG、IgA水平存在一定的相关性,其中抗ds-DNA抗体、C3 与IL-35,IgG与CCL19 间存在一定的影响关系.
Abstract
Objective:To observe the changes of serum interleukin-35(IL-35)and C-C motif chemo-kine ligand 19(CCL19)in children with systemic lupus erythematosus(SLE),and to analyze the association with immunological indexes.Methods:A total of 97 children with SLE in the hospital were included from January 2022 to March 2024,and 97 healthy children were selected for control study during the same time pe-riod.The changes of serum IL-35 and CCL19 levels were observed,and the correlation with immunological indexes[including anti-ds-DNA antibody,immunoglobulin(Ig),complements C3 and C4]was analyzed.Results:Compared with the healthy children,the children with SLE had a significantly lower serum IL-35 level and a significantly higher serum CCL19 level(P<0.05).Compared with the children with inactive SLE,serum levels of IL-35,C3,and C4 in the children with active SLE were significantly lower while serum levels of CCL19,anti-ds-DNA antibody,IgM,IgG,and IgA were significantly higher(P<0.05).Pearson correla-tion analysis showed that the serum level of IL-35 in children with SLE was negatively correlated with the lev-els of anti-ds-DNA antibody,IgM,IgG,and IgA,and was positively correlated with the levels of C3 and C4(P<0.05).Serum CCL19 level was positively correlated with the levels of anti-ds-DNA antibody,IgM,IgG and IgA,and was negatively correlated with the levels of C3 and C4 in children with SLE(r=-0.314,-0.203,-0.301,-0.201,0.318,0.383,P<0.05).Linear regression analysis suggested that anti-ds-DNA antibody had a negative impact on IL-35 level in children with SLE(r=0.272,0.209,0.362,0.202,-0.219,-0.391,P<0.05)while C3 had a positive impact on IL-35 level(P<0.05).IgG in children with SLE had a positive impact on CCL19 level(P<0.05).Conclusion:The abnormal changes of serum IL-35 and CCL19 levels in children with SLE are correlated with the levels of anti-ds-DNA antibody,IgM,IgG and IgA.There is a certain influence relationship between anti-ds-DNA antibody,C3 and IL-35,IgG and CCL19.
基金项目
新疆维吾尔自治区自然科学基金(2022D01C466)
维普
万方数据