Resina Draconis Extract Regulating HIF-1α/VEGF Signaling Pathway to Promote Wound Healing in Rats with Perianal Abscess
Objective:To analyze the effect of resina draconis extract on promoting wound healing in rats with perianal abscess by regulating HIF-1α/VEGF signaling pathway.Methods:Forty SPF grade SD rats were selected,with 10 as the control group and 30 used to construct the perianal abscess model.A total of 24 rats were successfully modeled and divided into a model group(8),a resina draconis extract group(8),and a resina draconis extract+HIF-1α inhibitor group(8).The control group and the model group were not trea-ted.The resina draconis extract group was treated with resina draconis extract coated on sterile gauze and cov-ered on the wound surface,while the resina draconis extract+HIF-1α inhibitor group was treated with resina draconis extract applied on the wound surface and injected with 10 μmoL/L HIF-1α inhibitor.After 14 days of administration,the pathological histology,wound healing,number of newly formed capillaries,fibroblast count,angiogenesis related factors,inflammatory factors,HIF-1α/VEGF pathway related mRNA expression levels,and HIF-1α/VEGF expression levels were observed in each group of rats.Results:Compared with the model group,the resina draconis extract group and the dragon's blood extract+HIF-1α inhibitor group had higher wound healing rates after 4 days,9 days,and 14 days of treatment,and the resina draconis extract group had a higher wound healing rate(P<0.05).Compared with the control group,the number of newly formed capillaries and fibroblasts in the model group decreased,while the levels of bFGF,EGF,HIF-1α,VEGF related mRNA expression,HIF-1α,and VEGF expression decreased.The levels of TNF-α,IL-6,and IL-β increased.Compared with the model group,the resina draconis extract group and the resina draco-nis extract+HIF-1α inhibitor group increased the number of newly formed capillaries and fibroblasts,while the levels of bFGF,EGF,HIF-1α,VEGF related mRNA expression,HIF-1α,and VEGF increased.The levels of TNF-α,IL-6,and IL-β decreased,and the number of newly formed capillaries and fibroblasts in the resina draconis extract group was higher than that in the resina draconis extract+HIF-1α inhibitor group.The levels of EGF,HIF-1α,VEGF related mRNA expression,HIF-1α,and VEGF expression were higher in the resina draconis extract+HIF-1α inhibitor group than in the resina draconis extract+HIF-1α in-hibitor group,the levels of TNF-α,IL-6,and IL-β were lower in the resina draconis extract+HIF-1α in-hibitor group(P<0.05).Conclusion:The intervention of resina draconis extract on perianal abscess rats can promote wound healing by regulating the HIF-1α/VEGF signaling pathway,and has a good therapeutic effect.
万方数据
维普
