Periplaneta americana extract CⅡ-3 inducing SKOV3 cells aging by regulating p16INK4a protein
Objective To investigate the effect of Periplaneta americana extract CⅡ-3 in regulating p16INK4a pro-tein in inducing SKOV3 aging.Methods SKOV3 cells were treated with different concentrations of Periplaneta americana extract CⅡ-3,and the effects of CⅡ-3 on the proliferation of SKOV3 cells were detected by CCK-8 assay.The effect of Periplaneta americana extract CⅡ-3 on the colony formation of SKOV3 cells was determined by colony culture experiments.SKOV3 cell cycle changes after CⅡ-3 action were examined with flow cytometry detection.Senescence changes in SKOV3 cells after CⅡ-3 action were determined with SA-β-Gal staining de-tects.Western Blotting was used to detect the effects of CⅡ-3 on SKOV3 cells phosphorylated p16INK4a protein expression.Results Periplaneta americana extract CⅡ-3 could effectively inhibit the proliferation of SKOV3 cells,and the optimal concentration was 80 μg/mL and an action time was 48 h.The colony formation ratio of SKOV3 cells were reduced after the effect of Periplaneta americana extract CⅡ-3 on SKOV3 cells,the proportion of cells blocked in G0/G1 phase were increased,the proportion of cells in S phase decreased,the percentage of positive cells of SA-β-Gal was increased and the upregulation of the expression of the aging regulatory molecule p16INK4a protein were raised.Conclusion Periplaneta americana extract CⅡ-3 could induce senescence in SK-OV3 cells by modulating protein expression of the senescence regulatory molecule p16INK4a.
Periplaneta americana extract CⅡ-3SKOV3 cellssenescencep16INK4a protein
NETL
NSTL
万方数据
