Study on dapagliflozin in improving cardiotoxicity induced by anthracyclines in mice with speckle track-ing technique
Objective To investigate the feasibility of speckle tracking technique in evaluating the improvement of cardiotoxicity induced by anthracyclines with dapagliflozin.Methods Male BALB/c mice were randomly divided into normal control group,epirubicin group and epirubicin plus dapagliflozin group.Dapagliflozin were adminis-tered at three consecutive days before epirubicin treatment,followed by once every other day for 3 weeks(10 mg/kg,p.o.).Epirubicin(3 mg/kg,i.p.)or an equal volume of 0.9%sodium chloride solution were injected every 4 days for 3 weeks.Conventional echocardiography and measurement of left ventricular global lon-gitudinal strain(GLS)based on speckle tracking technique were performed at baseline,one day after the third Epi dosage and one day after the last chemotherapy.Myocardial damage were evaluated by histological analysis at the end of experiment.Results Compared with control group and epirubicin plus dapagliflozin group,epirubi-cin group showed significant abnormalities in myocardial cell structure,and an increase in collagen content and percentage of myocardial cell apoptosis.In terms of cardiac function,GLS decreased significantly after the third injection in epirubicin group(P<0.05),and GLS continued to decrease with increasing cumulative dose(P<0.05).GLS only showed slight decrease in epirubicin plus dapagliflozin group after the last chemotherapy,but this difference was not statistically significant(P>0.05).In addition,there was a good correlation between GLS and myocardial pathological damage,with collagen content showing the highest correlation(rs=-0.637,P<0.001).Conclusion Dapagliflozin can significantly improve the heart damage caused by epirubicin in the mouse model.GLS is feasible for evaluating heart function improvement after receiving cardioprotective drugs.
NETL
NSTL
万方数据
