栀子与淡豆豉配伍减毒机制的研究
Study on the attenuated toxicity mechanism of the combination of gardenia and semen sojae praeparatum
杜玉青 1袁美杰 1王鑫国2
作者信息
- 1. 河北中医学院, 河北 石家庄 050200
- 2. 河北中医学院实验中心, 河北 石家庄 050200
- 折叠
摘要
目的 探讨栀子与淡豆豉配伍减毒作用的机制.方法 将50只Wistar大鼠随机分为空白对照组(等容积蒸馏水)、栀子中剂量组(1.4 g生药/kg)、栀子高剂量组(2.8 g生药/kg)、栀子中剂量+淡豆豉中剂量组(各1.4 g生药/kg)、栀子高剂量+淡豆豉高剂量组(各2.8 g生药/kg),连续灌胃给药7 d后,腹主动脉取血,测定大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性,将大鼠脊椎脱臼处死取出肝脏,计算肝脏指数并观察肝脏病理变化.结果 与空白对照组比较,栀子中剂量组、栀子高剂量组、栀子中剂量+淡豆豉中剂量组、栀子高剂量+淡豆豉高剂量组ALT、AST升高(P ＜0.05);栀子高剂量组ALT、AST高于栀子中剂量组(P ＜0.05);栀子中剂量+淡豆豉中剂量组、栀子高剂量+淡豆豉高剂量组与栀子中剂量组、栀子高剂量组比较ALT、AST均降低(P ＜0.05);栀子高剂量+淡豆豉高剂量组与栀子中剂量+淡豆豉中剂量组比较ALT、AST降低(P ＜0.05).与空白对照组比较,栀子中剂量组、栀子高剂量组、栀子中剂量+淡豆豉中剂量组、栀子高剂量+淡豆豉高剂量组肝脏指数均升高(P ＜0.05);栀子高剂量组肝脏指数高于栀子中剂量组(P ＜0.05);栀子高剂量+淡豆豉高剂量组与栀子中剂量+淡豆豉中剂量组比较肝脏指数降低(P ＜0.05);与栀子中剂量组、栀子高剂量组比较,栀子中剂量+淡豆豉中剂量组、栀子高剂量+淡豆豉高剂量组肝脏指数均降低(P ＜0.05).结论 栀子与淡豆豉配伍使用,能够大大降低肝毒性.
Abstract
Objective To investigate the attenuated toxicity mechanism of the combination of gardenia and semen sojae praeparatum. Methods 50 Wistar rats were randomly divided into blank control group (equal volume distilled water), gardenia medium dose group (1. 4 g crude drug/kg), gardenia high dose group (2. 8 g crude drug/kg), gardenia medium dose plus semen sojae praeparatum medium dose group (1. 4 g crude drug/kg each), gardenia high dose plus semen sojae praeparatum high dose group (2. 8 g crude drug/kg each). After 7 days of continuous gavage, abdominal aorta blood of rats was taken to determine the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, the liver was removed from the dislocated vertebrae of rats, and the liver index was calculated and the pathological changes of liver were observed. Results Compared with the blank control group, the ALT and AST of gardenia medium dose group, gardenia high dose group, gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group were increased (P ＜ 0. 05). The ALT and AST in the high dose group of gardenia were higher than those in the medium dose group of gardenia (P ＜ 0. 05). The ALT and AST of gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group were lower than that of gardenia medinm dose group and gardenia high dose group (P ＜0. 05). The ALT and AST of gardenia high dose plus semen sojae praeparatum high dose group were lower than that of gardenia medium dose plus semen sojae praeparatum medium dose group (P ＜ 0. 05). Compared with the blank control group, the liver index increased in gardenia medium and high dose group, gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group (P ＜ 0. 05).The liver index of the high dose group of gardenia was higher than that of the middle dose group of gardenia (P ＜ 0.05). The liver index of gardenia high dose plus semen sojae praeparatum high dose group was lower than that of gardenia medium dose plus semen sojae praeparatum medium dose group (P ＜ 0. 05). Compared with gardenia medium dose group and gardenia high dose group, the liver index of gardenia medium dose plus semen sojae praeparatum medium dose group andgardenia high dose plus semen sojae praeparatum high dose group were decreased (P ＜ 0. 05).Conclusion The combination of gardenia and semen sojae praeparatum can greatly reduce liver toxicity.
关键词
栀子/淡豆豉/毒性/中药配伍Key words
Gardenia/Semen sojae praeparatum/Toxicity/Compatibility of traditional Chinese引用本文复制引用
基金项目
2015年全国大学生创新创业训练计划项目(201514432007)
出版年
2019
国家科技期刊平台
NSTL
维普
万方数据