Regulatory effects of Amentoflavone on the proliferation,invasion,migration and adhesion of human gastric cancer cells and the underlying mechanism
Objective To investigate the regulatory effects of Amentoflavone(AMF)on the proliferation,invasion,mi-gration and adhesion of human gastric cancer cells in vitro,and its underlying mechanism.Methods The human gastric cancer cell BGC-823 was used in the following experiments.The regulatory effects of AMF on the proliferation,invasion,migration and adhe-sion of BGC-823 cells were detected by methyl thiazolyl tetrazoli-um(MTT)assay,Transwell assay,wound healing assay and cell adhesion assay,respectively.Protein expressions of those associat-ed with cell invasion,migration and adhesion,including the matrix metalloproteinase-2(MMP-2),MMP-9 and reversion-inducing-cysteine-rich protein with kazal motifs(RECK)were detected by Western blot.Similarly,protein expressions of those involved in the PI3K/Akt signaling pathway,including the phosphatidylinositol 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(Akt)and phosphorylated Akt(p-Akt)were detected by Western blot as well.Results The MTT assay showed that the treatment of AMF at 50,100,200 and 400 μmol/L significantly inhibited proliferation in BGC-823 cells compared with those induced with 1‰dimethyl sulfoxide(DMSO)or blank control(P＜0.05).The inhibitory rate of proliferation dose-dependently increased with the increase in the concentration of AMF(P＜0.05).Transwell assay showed that the number of invasive cells in BGC-823 cells induced with 100,200 and 400 μmol/L was significantly less than those treated with blank control(P＜0.05),which was in a dose-de-pendent manner(P＜0.05).Wound healing assay showed that the number of migratory cells in BGC-823 cells induced with 100,200 and 400 μmol/L was significantly less than those treated with blank control(P＜0.05),which was in a dose-de-pendent manner(P＜0.05).Cell adhesion assay showed that the number of adhesive cells treated with 100,200 and 400 μmol/L for 20,40,60 and 80 min on the fibronectin gel was significantly less than those induced with blank control(P＜0.05),which was in a dose-dependent and time-dependent manner(P＜0.05).Western blot showed that after the treatment of 100,200 and 400 μmol/L AMF,RECK was significantly upregulated,and MMP-2 and MMP-9 were significantly downreg-ulated than those treated with blank control(P＜0.05).Their protein expressions all presented the dose-dependent changes(P＜0.05).Compared with those of blank control,p-AKT and p-PI3K were significantly downregulated in BGC-823 cells induced with 100,200 and 400 μmol/L AMF in a dose-dependent manner(P＜0.05).Conclusion AMF can effectively in-hibit the proliferation,invasion,migration and adhesion of human gastric cancer cells by inhibiting the PI3K/Akt signaling path-way via upregulating RECK,and downregulating MMP-2,MMP-9,p-PI3K and p-AKT.
Gastric cancerExperimentAmentoflavonePharmacology of Chinese medicine
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维普
