Mechanism of Radix Actinidiae chinensis in treating colorectal cancer based on the network pharmacology
Objective To explore the therapeutic mechanism of the traditional Chinese medicine(TCM)herbal Radix Actinidiae chinensis in the treatment of colorectal cancer(CRC)based on the network pharmacology.Methods The main ac-tive ingredients and drug targets of Radix Actinidiae chinensis were obtained using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and the Pharmacology-based Network Computational Research Platform of Traditional Chinese Medicine(TCMIP).Disease targets of CRC were predicted using the GeneCards database and the Online Mendelian Inheritance in Man(OMIM).The intersection of drug targets and disease targets was obtained using the R package and visualized in a Venn diagram,which were therapeutic targets of Radix Actinidiae chinensis in the treatment of CRC.Cyto-scape3.6.1 software and String database were used to construct the drug-component-disease-target network and protein-protein interaction(PPI)network.Finally,therapeutic targets of Radix Actinidiae chinensis in the treatment of CRC were subjected to Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Ge-nomes(KEGG)enrichment analyses,and visualized using the Database for Annotation,Visualization and Integrated Discovery(DAVID).Results A total of 6 active ingredients and 154 disease targets of Radix Actinidiae chinensis,and 10 622 disease targets associated with CRC were screened.By making an intersection,145 therapeutic targets of Radix Actinidiae chinensis in the treatment of CRC were obtained.The six active ingredients of Radix Actinidiae chinensis,including aloe-emodin,ent-Epicatechin,(+)-catechin,quercetin,sitosterol and beta-sitosterol were all the core targets for the treatment of CRC.Key targets included AKT1(AKT Serine/Threonine Kinase 1),IL-6(interleukin 6),VEGFA(Vascular Endothelial Growth Fac-tor A),JUN(Jun proto-oncogene),CASP3(caspase-3),IL-1β(interleukin 1β),EGFR(epidermal growth factor recep-tor)and ESR1(estrogen receptor 1).GO enrichment analysis obtained 141 terms of biological processes(BP),mainly inclu-ding the DNA-binding transcription factor activity,transcription factor activity,cytokine activity and heme-binding antioxidant activity.The KEGG pathway enrichment analysis showed that the core targets were mainly enriched in the AGE-RAGE(Ad-vanced Glycation End Products and receptor for AGEs),TNF(tumor necrosis factor),IL-17(interleukin 17)and HIF-1(Hypoxia-Inducible Factor 1)signaling pathways.Conclusion Based on the network pharmacology,we primarily explore and verifie the multi-component,multi-target and multi-pathway therapeutic regulation of Radix Actinidiae chinensis in CRC,predict the potential of Radix Actinidiae chinensis in the treatment of CRC,and provide scientific references for analyzing its ac-tive ingredients and experimental research.
Radix Actinidiae chinensisColorectal cancerPharmacology of chinese medicineMechanism
万方数据
维普
