Antioxidationeffect of Bombyx Batryticatus Polysaccharide on mice with type 2 diabetes mellitus
Objective To investigate the antioxidant effect of Bombyx Batryticatus Polysaccharide(BBP)on mice with type 2 diabetes mellitus(T2DM).Methods A total of 30 Kunming male mice in the specific pathogen-free(SPF)level were used.Seven randomly selected mice were treated with blank control(control group),and the remaining 23 were subjected to the modeling of T2DM.Two mice died during the modeling period.Twenty-one T2DM mice were randomly divided into the model group,BBP group and metformin group,with 7 mice per group.Mice in the BBP group and metformin group were administrated with 400 mg/kg BBP solution and 200 mg/kg metformin solution by oral gavage once daily for 28 days,respectively.Mice in the model group and control group were similarly given 0.2 mL/10 g 0.9%NaCl solution.Pathological changes in mouse liver were ob-served.Body mass,fasting plasma glucose(FPG),organ indexes(spleen index,kidney index)and oxidation stress indexes(total superoxide dismutase[T-SOD],malondialdehyde[MDA]and glutathione[GSH])were compared.Results Mice in the control group presented a regular arrangement of liver cells with a clear margin,and edema and inflammation were not observed.Mice in the model group presented an irregular arrangement,dif-fuse edema degeneration and mild steatosis in the majority of liver cells,loose cellular tissue,severe vacuolization with many small round vacuoles of varying sizes,and mild inflammation.Mice in the BBP group presented uniform and completely-shaped liver cells,slightly loose cellular tissues,a small number of small round vacuoles of varying sizes on cell surface,mild edema,steatosis and no inflammation.Mice in the metformin group presented a regular arrangement of liver cells,mild vacuolization,moderate cell body swelling and no inflammation.Compared with those of the control group,mice in the model group presented significantly lower body mass and higher FPG(both P<0.05).Compared with those of the model group,mice in the BBP group and metformin group presented significantly higher body mass and lower FPG(both P<0.05).There were no significant differ-ences in the body mass and FPG between BBP group and metformin group(P>0.05).Compared with those of the control group,mice in the model group presented significantly higher spleen index and kidney index(P<0.05).Compared with those of the model group,mice in the BBP group and metformin group presented significantly lower spleen index and kidney index(P<0.05).There were no significant differences in the spleen index and kidney index between BBP group and metformin group(P>0.05).Compared with those of the control group,mice in the model group presented significantly lower T-SOD and GSH and higher MDA(both P<0.05).Compared with those of the model group,mice in the BBP group and metformin group presen-ted significantly higher T-SOD and GSH and lower MDA(both P<0.05).There were no significant differences in the T-SOD,GSH and MDA between BBP group and metformin group(P>0.05).Conclusion BBP can reduce hepatic steatosis and enhance its antioxidant effect on T2DM mice,suggesting that the antioxidant BBP lowers blood glucose in T2DM mice by regula-ting oxidative stress.
Type 2 diabetes mellitusBombyx BatryticatusAntioxidationAnimal experiment
万方数据
维普
