Effects of electroacupuncture on chronic heart failure rat model in vivo and myocardial fibrosis model in vitro
Objective To observe the effects of electroacupuncture(EA)on chronic heart failure(CHF)rat model in vivo and myocardial fibrosis model in vitro,and to explore the therapeutic mechanism of electroacupuncture for CHF.Methods Twenty-four male Wistar rats were randomly divided into 4 sham operation group,sham operation + EA group,myocardial fibrosis group,and myocardial fibrosis + EA group,with 6 rats in each group.Transverse aortic coarctation was performed for CHF model-ing in rats,and those in sham operation group were managed by i-solation of the abdominal aorta without clamping.Electroacupunc-ture at the Neiguan acupoint on bilateral sides was performed.Rat cardiomyocyte cell line H9c2 was induced with blank control,control + EA,angiotensin Ⅱ,and angiotensin Ⅱ + EA.H9c2 cells induced with blank control were routinely cultured and passaged.Angiotensin Ⅱ and electric stimulations were intervened in vitro.At 12,16 and 20 weeks,rat interventricular septal thickness(IVSd),left ventricular posterior wall thickness(LVPWd),left ventricular end-systolic dimension(LVEDs),left ventricular end-diastolic dimension(LVEDd)and left ventricular ejection fraction(LVEF)were recorded.Serum B-type na-triuretic peptide(BNP),lactate dehydrogenase(LDH)and creatine kinase(CK)were measured.At 12,16 and 20 weeks,relative levels of matrix metalloproteinase 9(MMP9)and tissue inhibitor of metalloproteinases 1(TIMP-1)in H9c2 cells were detected.Results Compared with rats of sham operation group,those in myocardial fibrosis group had significantly higher IVSd,LVPWd,LVEDs and LVEDd at 12,16 and 20 weeks,but lower LVEF(P<0.05).There were no significant differences in IVSd,LVPWd,LVEDs,LVEDd and LVEF between sham operation group and sham operation + EA group(P>0.05).Compared with rats of myocardial fibrosis group,those in myocardial fibrosis + EA group had significantly lower IVSd,LVPWd,LVEDs and LVEDd at 12,16 and 20 weeks,but higher LVEF(P<0.05).Compared with rats of sham operation group,those in myocardial fibrosis group had significantly higher BNP,LDH and CK levels at 12,16 and 20 weeks(P<0.05).There were no significant differences in BNP,LDH and CK between sham operation group and sham operation + EA group(P>0.05).Compared with rats of myocardial fibrosis group,those in myocardial fibrosis + EA group had significantly lower BNP,LDH and CK(P<0.05).Compared with H9c2 cells induced with blank control,significantly upregulated MMP-9 and TIMI-1 were detected in angiotensin Ⅱ-induced cells at 12,16 and 20 weeks(P<0.05).No significant differences were detected in the expression levels of MMP-9 and TIMI-1 between H9c2 cells induced with blank control and control + EA(P>0.05).Com-pared with H9c2 cells induced with angiotensinⅡ,treatment of angiotensinⅡand EA significantly downregulated MMP-9 and TIMI-1at 12,16 and20 weeks(P<0.05).Conclusion The therapeutic effect of EA on CHF is attributed to the inhibition of ventricular remodeling,improvement of cardiac function,protection of myocardial cells,and alleviation of myocardial fibrosis.
Heart failureMyocardial fibrosisElectroacupuncture therapyIn vivo experiments,In vitro experiments
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