Effects of Guizhi Fuling Capsules Combined with goserelin on Aldo-keto reductase family 1 member C3 and endocrine in a rat model of uterine leiomyomas
Objective To explore the effects of Guizhi Fuling Capsules combined with goserelin on Aldo-keto reduc-tase family 1 member C3(AKR1C3)and endocrine in a rat model of uterine leiomyomas.Methods Thirty-six 7-week-old female interleukin-2 receptor gamma chain gene(IL2RG)-deficient rats were randomly divided into blank control group,mod-el group and combination group,with 12 rats per group.Rats in the latter two groups were modeling for uterine leiomyomas by medications of estrogen and progesterone drugs.On the second day after the modeling,rats in the blank control group and model group were given oral gavage of 5 mL of distilled water,those in the combination group were given oral gavage of Guizhi Fuling Capsule suspension(0.70 g/kg/d)+goserelin(1.25 mg/kg/d)for consecutive 6 weeks.Enzyme-linked immunosorbent as-say(ELISA)was used to measure serum estradiol,progesterone and prolactin levels.Quantitative real-time polymerase chain reaction(QRT-PCR)was performed to detect the mRNA level of AKR1C3.Western blotting was used to detect protein expres-sions of collagen type Ⅰ(Col-Ⅰ),transforming growth factor β(TGF-β),plasminogen activator inhibitor-1(PAI-1),caspase-3,B-cell lymphoma 2(Bcl-2)and Bcl-2-related X protein(Bax).Results The levels of serum estradiol,progesterone and prolactin in the model group were significantly higher compared with the blank control group(P<0.05),and which in the combination group were significantly lower compared to the model group(P<0.05).The mRNA level of AKR1C3 was significantly higher in the model group relative to the blank control group(P<0.05),and which was significantly lower in the combination group than that of the model group(P<0.05).The protein levels of Col-I,TGF-β and PAI-1 were significantly higher in the model group versus the blank control group(P<0.05),and which were significantly lower in the combination group versus the model group(P<0.05).Compared with blank control group,the protein level of Caspase-3 and Bax in the model group was significantly lower,and that of Bcl-2 was signifi-cantly higher(P<0.05).Compared with the model group,the protein levels of Caspase-3 and Bax in the combination group were significantly higher,and the protein level of Bcl-2 was significantly lower(P<0.05).Conclusion Guizhi Fuling Cap-sules combined with goserelin can reduce serum estradiol,progesterone and prolactin,inhibit the expression of AKR1C3,sup-press the proliferation and stimulate the apoptosis in rats,showing the inhibitory effect on the growth of uterine leiomyomas.
LeiomyomasRatsAnimal modelGuizhi Fuling CapsulesGoserelinXenograftAldo-keto reductase family 1 member C3
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