Effect of optimized Kuijie Prescription on colon tissue in ulcerative colitis rats by regulating TLR/MyD88/NF-κB sig-naling pathway
Objective To assess the effect of optimized Kuijie Prescription on colon tissue in ulcerative colitis(UC)rats by regulating Toll-like receptor(TLR)/myeloid differentiation factor 88(MyD88)/nuclear factor kappa B(NF-κB).Methods A total of 40 male Sprague-Dawley(SD)rats were randomly divided into normal group,model group,optimized Kuijie Pre-scription group and sulfasalazine group,with 10 rats in each group.Except for the blank group,the other three groups were treated with trinitrobenzene sulfonic acid(TNBS)/ethanol second-ary inflammation method combined with restraint method to estab-lish UC rat model of qi stagnation and blood stasis type.After successful modeling,a 14-day intervention was conduted in Kui-jie Prescription group(1.674 g/kg/d optimized Kuijie Prescription),sulfasalazine group(0.54 g/kg/d sulfasalazine),blank group and model group(water administration)to detect serum levels of interleukin-17A(IL-17A),IL-10,IL-22,and the protein levels of MyD88,TLR2,TLR4 and NF-κB p65 and the expression of mRNA in colon tissue of rats.Results Com-pared with blank group,the levels of IL-17A and IL-22 in model group were significantly increased(P<0.05),while the level of IL-10 was significantly decreased(P<0.05).Compared with model group,the serum levels of IL-17A and IL-22 in optimized Kuijie Prescription group and sulfasalazine group were significantly decreased(P<0.05),but the serum level of IL-10 was significantly increased(P<0.05).There was no significant difference in the serum levels of IL-17 A,IL-22 and IL-10 between optimized Kuijie Prescription group and sulfasalazine group(P>0.05).Compared with blank group,the pro-tein levels of MyD88,TLR2,TLR4,NF-κB p65 and the expression of mRNA in colon tissue in model group were significantly increased(P<0.05).Compared with model group,the protein levels of MyD88,TLR2,TLR4,NF-κB p65 and the expres-sion of mRNA in sulfasalazine group and optimized Kuijie Prescription group were significantly decreased(P<0.05).There was no significant difference in the protein levels of MyD88,TLR2,TLR4,NF-κB p65 and the expression of mRNA between opti-mized Kuijie Prescription group and sulfasalazine group(P>0.05).Conclusion Optimized Kuijie Prescription may reduce the serum level of inflammatory factors,improve the inflammatory response,and repair the ultrastructure of colon tissue by regulating the TLR/MyD88/NF-κB signaling pathway,thereby repairing the colonic mucus barrier function in UC rats.
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