Wusangyin medicated serum alleviates high-glucose-induced podocyte injury through the SIRT1/AMPK signaling pathway and enhancing autophagy
Objective To explore the protective role of Wusangyin medicated serum in podocyte injury in vitro and the underlying mechanism.Methods Sprague-Dawley(SD)rats were managed by Wusangyin for 7 days via oral gavage,and Wusangyin medicated serum of rats was prepared.High-glucose-induced mouse podocytes were treated with Wusangyin medi-cated serum from modeling rats and serum from controlled rats for 48 hours,and podocytes treated with low glucose and high glu-cose were used as control group and model group,respectively.Cell viability,apoptosis rate,supernatant concentrations of lac-tate dehydrogenase(LDH),tumor necrosis factor alpha(TNF-α)and interleukin 1β(IL-1β),and malondialdehyde(MDA)content and superoxide dismutase(SOD)activity in cell lysates were measured.Autophagy was measured by immuno-fluorescence.Quantitative polymerase chain reaction(qPCR)was used to detect gene expressions.Protein levels of SIRT1 and AMPK were analyzed by Western blot.Results Treatment of Wusangyin medicated rat serum significantly enhanced the prolifer-ation of podocytes,and inhibited supernatant concentration of LDH,apoptotic rate,inflammatory factor levels and oxidative stress levels.Wusangyin medicated serum further promoted high-glucose-induced autophagy,increased the mRNA level of Beclin-1 and decreased the mRNA level of p62,as well as upregulated SIRT1 and p-AMPK in podocytes.Conclusion Wusangyin medicated serum can promote podocyte autophagy and reduce podocyte injury induced by high glucose by activating the SIRT1/AMPK pathway.
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