冬凌草甲素介导Notch信号通路对多发性骨髓瘤细胞生长水平、凋亡率及异常转化情况研究
Study on the growth leveland apoptotic rate and abnormal transformation of multiple myeloma cells by oridonin-medi-ated Notch signaling pathway
祝龙 1詹其林 2黄菊 2方婷婷 2潘显阳2
作者信息
- 1. 上海市第六人民医院金山分院血液肿瘤科,上海 201599
- 2. 上海市公共卫生临床中心血液科,上海 200093
- 折叠
摘要
目的 探究冬凌草甲素介导Notch信号通路对多发性骨髓瘤细胞生长水平、凋亡率及异常转化情况.方法 选取U266 细胞、RPMI8226 细胞和KAS-6/1 细胞,采用CCK8 法检测细胞生长水平,采用酶标仪 492 nm处检测光密度检测物厚度D值测定细胞的生长抑制率,随后采用流式细胞仪检测RPMI8226 细胞和 U266 细胞的凋亡情况,最后对所检测细胞进行异常转化的观察.结果 研究组U266 细胞、RPMI8226 细胞和KAS-6/1 细胞的生长水平均明显低于对照组(P<0.05).研究组干预后Notch 1-siRNA与核因子κB(NF-κB)-siRNA表达水平变化明显优于对照组(P<0.05).研究组RPMI8226 细胞、U266 细胞凋亡率明显优于对照组(P<0.05).研究组异常转染、异常克隆、异常增生的发生率明显低于对照组(P<0.05).结论 冬凌草甲素能够明显抑制多发性骨髓瘤 U266 细胞、RPMI8226 细胞、KAS-6/1 细胞体外生长,有效激活Notch信号通路调节诱导肿瘤细胞凋亡过程,值得临床推广应用.
Abstract
Objective To investigate the effects of oridonin-mediated Notch signaling pathway on the growth level,ap-optosis rate,and abnormal transformation of human multiple myeloma cells.Methods U266 cells,RPMI8226 cells and KAS-6/1 cells were induced with blank control or oridonin.Cell viability was measured by CCK-8 assay.Cell growth inhibition was meas-ured by detecting optical density at 492 nm wavelength using a microplate reader.Flow cytometry was performed to examine the ap-optosis of RPMI8226 and U266 cells.Abnormal transformation of the detected cells was observed.Results Cell growth was signifi-cantly lower in U266 cells,RPMI8226 cells and KAS-6/1 cells treated with oridonin than those of controls(P<0.05).Expres-sion levels of Notch 1 and NF-κB siRNA were significantly upregulated in U266 cells,RPMI8226 cells and KAS-6/1 cells trea-ted with oridonin than those of controls(P<0.05).A higher apoptotic rate was detected in oridonin-induced RPMI8226 and U266 cells in comparison to controls(P<0.05).The incidences of abnormal transfection,colony formation and proliferation were significantly lower in U266 cells,RPMI8226 cells and KAS-6/1 cells treated with oridonin than those of controls(P<0.05).Conclusion Oridonin can significantly inhibit the growth of U266 cells,RPMI8226 cells and KAS-6/1 cells in vitro,and effec-tively activate Notch signaling pathway and induce apoptosis of tumor cells,which is worthy of clinical application.
关键词
冬凌草甲素/多发性骨髓瘤/Notch信号通路/细胞凋亡Key words
Oridonin/Multiple myeloma/Notch signal path/Cell apoptosis引用本文复制引用
基金项目
金山区卫生和计划生育委员会科研课题计划(JSKJ-KTMS-2018-02)
出版年
2024
国家科技期刊平台
NSTL
万方数据