Study on the growth leveland apoptotic rate and abnormal transformation of multiple myeloma cells by oridonin-medi-ated Notch signaling pathway
Objective To investigate the effects of oridonin-mediated Notch signaling pathway on the growth level,ap-optosis rate,and abnormal transformation of human multiple myeloma cells.Methods U266 cells,RPMI8226 cells and KAS-6/1 cells were induced with blank control or oridonin.Cell viability was measured by CCK-8 assay.Cell growth inhibition was meas-ured by detecting optical density at 492 nm wavelength using a microplate reader.Flow cytometry was performed to examine the ap-optosis of RPMI8226 and U266 cells.Abnormal transformation of the detected cells was observed.Results Cell growth was signifi-cantly lower in U266 cells,RPMI8226 cells and KAS-6/1 cells treated with oridonin than those of controls(P<0.05).Expres-sion levels of Notch 1 and NF-κB siRNA were significantly upregulated in U266 cells,RPMI8226 cells and KAS-6/1 cells trea-ted with oridonin than those of controls(P<0.05).A higher apoptotic rate was detected in oridonin-induced RPMI8226 and U266 cells in comparison to controls(P<0.05).The incidences of abnormal transfection,colony formation and proliferation were significantly lower in U266 cells,RPMI8226 cells and KAS-6/1 cells treated with oridonin than those of controls(P<0.05).Conclusion Oridonin can significantly inhibit the growth of U266 cells,RPMI8226 cells and KAS-6/1 cells in vitro,and effec-tively activate Notch signaling pathway and induce apoptosis of tumor cells,which is worthy of clinical application.
OridoninMultiple myelomaNotch signal pathCell apoptosis
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