Effects of dandelion root extract on the malignant behaviors of the breast cancer cell line MCF-7 and the Akt/HIF-1α signaling pathway
Objective To investigate the effect of dandelion root extract on the migration,invasion,proliferation and the Thr protein kinase B(Akt)/hypoxia-inducible factor-1 a(HIF-1α)signaling pathway in the breast cancer cell line MCF-7.Methods Human breast cancer cell line MCF-7 was cultured in vitro,and induced with DMSO(negative control)and dandelion root extract at varying concentrations(25,50,100,200 and 400 µg/mL).Cells were cultured in 2 mL of fresh medium containing DMSO or dandelion root extract.Cell migration,invasion and viability were detected by wound healing assay,Transwell assay and MTT assay,respectively.Expression levels of proteins involved in the Akt/HIF-1α signaling pathway were determined by Western blot.Results The wound healing assay data showed that 12-h and 24-h wound healing rate in MCF-7 cells induced with dandelion root extract at varying concentrations were significantly reduced in a dose-dependent manner than those induced with DMSO(P＜0.05),and the 24-h wound healing rate in each group was significantly higher than the 12-h value(P＜0.05).Transwell assay data showed that the invasive cell number in MCF-7 cells induced with dandelion root extract at varying concentrations was significantly reduced in a dose-dependent manner than those induced with DMSO(P＜0.05),which was not changed after the dose increased to 400 µg/mL(P＞0.05).MTT assay showed no significant difference in the 12-h viability among groups(P＞0.05),but significantly lower in MCF-7 cells induced with dandelion root extract at varying concentrations in a dose-dependent manner(P＜0.05).The 24-h viability in each group was significantly lower than the 12-h value(P＜0.05).Western blot showed significantly downregulated p-Akt and HIF-1α in MCF-7 cells induced with dandelion root extract at varying concentrations in a dose-dependent manner(P＜0.05).Conclusion Dandelion root extract inhibits the malignant biological behaviors of breast cancer MCF-7 cells by inhibiting the Akt/HIF-1 α signaling pathway.
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