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不同产地、不同部位及不同直径的黑老虎根质量差异研究

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目的 研究不同产地、不同部位及不同直径的黑老虎根质量差异.方法 采用UPLC法,测定14批不同产地、不同部位及不同直径黑老虎根的指纹图谱,结合相似度评价、单因素方差分析、聚类分析及灰色关联度分析法,对各黑老虎根样品的指纹图谱进行多元统计分析.结果 黑老虎根UPLC指纹图谱标定了8个共有峰,指认了峰5为8-姜酚.不同产地、不同部位及不同直径的黑老虎根部分样品指纹图谱与对照指纹图谱的相似度较低.不同批次黑老虎根药材与木质部、皮部的共有峰的单位峰面积波动较大,不同直径的黑老虎根样品间的共有峰的单位峰面积差异明显.聚类分析结果显示,不同批次的黑老虎根药材无明显的产地分类聚集现象;灰色关联度分析结果显示,不同批次黑老虎根药材灰色关联度综合评价值范围为0.066~0.094,灰色关联度差异范围为0~29.79%.结论 市场上的黑老虎根药材内在质量差异较大,建立的UPLC指纹图谱检测方法重复性与稳定性良好,可为黑老虎根的质量评价提供参考.
Study on the quality differences of Kadsura coccinea in different habitats,different parts and different diameters
Objective To investigate the difference of quality of Kadsura coccinea in different habitats,different parts and different diameters.Methods The fingerprints of 14 batches of Kadsura coccinea in different habitats,different parts and different diameters were determined by UPLC method,and combined with similarity evaluation,one-way ANOVA,cluster analysis,and grey correlation analysis,multivariate statistical analysis was conducted on the fingerprint of Kadsura coccinea.Results Eight common peaks were identified in the samples of Kadsura coccinea in different habitats,different parts and different diameters,and the peak 5 was identified as 8-gingerol.The similarity between the fingerprint of Kadsura coccinea in different habitats,different parts and different diameters was relatively low.The unit peak area of common peaks between different batches of Kadsura coccinea and the xylem and skin fluctuated greatly,and there was a significant difference in the unit peak area of common peaks between Kadsura coccinea of different diameters.The clustering analysis results showed that there was no obvious clustering phenomenon of origin classification for different batches of Kadsura coccinea;the grey correlation analysis results showed that the comprehensive evaluation value range of grey correlation degree for different batches of Kadsura coccinea was 0.066~0.094,and the difference range of grey correlation degree was 0~29.79%.Conclusion There is a significant difference in the internal quality of Kadsura coccinea circulating in the market.The UPLC fingerprint detection method established in this study has good repeatability and stability,which can provide reference for the quality evaluation of Kadsura coccinea.

Kadsura coccineaUPLC fingerprintGrey relational analysisQuality evaluation

陈巧华、杨赞、邓怡芳、周颖欣、李美洲、黄醒鹏、徐杰

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广东一方制药有限公司/广东省中药配方颗粒企业重点实验室,广东佛山 528244

黑老虎根 UPLC指纹图谱 灰色关联度法 质量评价

国家工信部产业技术基础公共服务平台项目-中药全产业链质量技术服务平台项目(2022)

2022-230-221

2024

湖北中医药大学学报
湖北中医药大学

湖北中医药大学学报

CSTPCD
影响因子:0.873
ISSN:1008-987X
年,卷(期):2024.26(1)
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