Effect of Tongdu Anshen Prescription on p-JNK,p-p38 protein expression in prefrontal lobe and serum TNF-α content in depression model rats
Objective To observe the effects of Tongdu Anshen Prescription on p-JNK,p-p38 protein expression in prefrontal lobe and serum TNF-α content in depression model rats,and to explore the anti-depressant mechanism of Tongdu Anshen Prescription.Methods Fifty rats were randomly divided into 5 groups,namely blank group,model group,Anshen group,Tongdu group and Tongdu Anshen group,10 rats each.A 21-day CUMS modelling approach was used to prepare a depressed rat model.After the modelling was successful,the rats were administered drugs and assessed again for behavioural changes.At the end of assessment,Westen Blot was used to detect p-JNK and p-p38 protein contents;ELISA was used to detect serum tumor necrosis factor-α(TNF-α)content;HE staining was used to observe the pathological morphology of prefrontal cells.Results Compared with blank group,model group showed decreased total distance,increased resting time in the open field(P＜0.05 or P＜0.01),significantly decreased sugar-water preference rate(P＜0.01),significantly enhanced prefrontal p38 and JNK protein phosphorylation expression levels(P＜0.01),significantly increased serum TNF-α(P＜0.01),and increased number of neuronal apoptosis.Compared with model group,the total distance of the open field was increased,the resting time was shortened(P＜0.05),and the sugar-water preference rate was significantly increased(P＜0.01)in each treatment group;p-p38 and p-JNK were decreased(P＜0.05),serum TNF-α levels were significantly decreased(P＜0.01),and the number of prefrontal neuronal cells was increased in both Anshen and Tongdu Anshen groups.Conclusion Tongdu Anshen Prescription can significantly improve the behavior of CUMS rats,reduce the level of serum TNF-α,effectively regulate the activation of JNK/p38 MAPK pathway,reduce the expression level of p38 and JNK phosphorylation in the prefrontal lobe of rats,while repairing damaged cells in the prefrontal lobe and exerting antidepressant effect.
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