Impact of oleuropein on chemotherapy resistance of endometrial cancer by regulating the RhoA/ROCK signaling pathway
Objective To investigate the impact of oleuropein on chemotherapy resistance of endometrial cancer and the role of RhoA/ROCK signaling pathway.Methods Endometrial cancer cells Ishikawa were cultured in vitro to establish a cisplatin resistant(DDP)cell line of Ishikawa cells.They were grouped into control group,DDP group,oleuropein-L+DDP group,oleuropein-M+DDP group,and oleuropein-H+DDP group;CCK8 experiment was applied to detect the inhibition rate of lshikawa/DDP cell proliferation in each group;flow cytometry was applied to detect the apoptosis rate of Ishikawa/DDP cells in each group;RT-PCR experiments were applied to detect the levels of RhoA and ROCK1 mRNA in Ishikawa/DDP cells in each group;Western Blot was applied to detect the levels of RhoA,ROCK1,Cleaved-Caspase3/Caspase3,MMP2,and Ki67 proteins in Ishikawa/DDP cells in each group.Results Compared with control group,there was no obvious difference in various indicators of Ishikawa/DDP cells in DDP group(P＞0.05);RhoA,ROCK1 mRNA levels,RhoA,ROCK1,MMP2,and Ki67 protein levels in oleuropein-L,M,H+DDP groups were obviously reduced;the proliferation inhibition rate,apoptosis rate,and the levels of Cleaved-Caspase3/Caspase3 were obviously increased,and were dose-dependent(P＜0.05).Conclusion Oleuropein may enhance the sensitivity of endometrial cancer resistant cell lines Ishikawa/DDP to DDP by inhibiting the RhoA/ROCK signaling pathway,thereby reducing DDP chemotherapy resistance in endometrial cancer.
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