Protective effect of Huanglian Jiedu Decoction on vascular endothelial injury in atherosclerotic rats by regulating ROS/NLRP3 pathway
Objective To investigate the effects of Huanglian Jiedu Decoction on vascular endothelial injury and reactive oxygen species(ROS)/nucleotide-binding oligomeric domain-like receptor protein 3(NLRP3)pathway in atherosclerosis(AS)rats.Methods An AS rat model were established and the experimental rats were divided into control group,model group,low,medium,high dose Huanglian Jiedu Decoction groups,and high-dose Huanglian Jiedu Decoction+ROS activator trimethylamine oxide(TMAO)group;fully automated biochemical analyzer was applied to detect the levels of total cholesterol(TC),triacylglycerol(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),and nitric oxide(NO)in serum;ELISA was applied to detect levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),IL-1β,superoxide dismutase(SOD),malondialdehyde(MDA),reactive oxygen species(ROS),and endothelin-1(ET-1);HE staining was applied to detect pathological and morphological changes in the thoracic aorta of rats;Western Blot was applied to detect the expression levels of NLRP3 and Caspase-1.Results Compared with control group,model group had severe endothelial damage to the aorta,thickening of the vascular wall and intima,and a large number of lipid plaques deposition;TC,TG,LDL-C,MDA,TNF-α,IL-6,IL-1β,ET-1 levels,ROS,NLRP3,and Caspase-1 expression were significantly increased;HDL-C,SOD,and NO levels were obviously decreased(P＜0.05);compared with model group,lipid plaques deposition in low,medium,and high dose Huanglian Jiedu Decoction groups gradually decreased,and the damage and thickening of the aortic endothelial cells gradually decreased;TC,TG,LDL-C,MDA,TNF-α,IL-6,IL-1β,ET-1 levels,ROS,NLRP3,and Caspase-1 expression were significantly reduced in sequence;HDL-C,SOD,and NO levels were significantly decreased in sequence(P＜0.05);compared with high dose Huanglian Jiedu Decoction group,high-dose Huanglian Jiedu Decoction+tri-methylamine oxide group showed increased endothelial thickening,damage to the aorta,and deposition of lipid plaques;TC,TG,LDL-C,MDA,TNF-α,IL-6,IL-1β,ET-1 levels,ROS,NLRP3,and Caspase-1 expression were obviously increased;HDL-C,SOD,and NO levels were significantly reduced(P＜0.05).Conclusion Huanglian Jiedu Decoction protects vascular endothelial injury in AS rats by inhibiting the ROS/NLRP3 pathway.
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