Identification of the Gleditsiae sinensis Semen and Gleditsiae japonica var.Delavayi Semen based on HPLC fingerprint
Objective To compare the differences between the Gleditsiae sinensis Semen and Gleditsiae japonica var.Delavayi Semen by HPLC fingerprint,so as to provide a basis for the identification of the Gleditsiae sinensis Semen by combining chemical chemometrics methods.Methods An Diasonsil C18(250 mm×4.6 mm,5 μm)chromatographic column was used,the mobile phase was 0.1％phosphoric acid solution and acetonitrile with gradient elution,the flow rate was 1.0 mL/min,column temperature was 25 degrees centigrade,and detection wavelength was 210 nm.The similarity evaluation software(2012A Version)of TCM chromatographic fingerprint prepared by pharmacopoeia committee was used for data processing,and the similarity between the extracts of the Gleditsiae sinensis Semen and Gleditsiae japonica var.Delavayi Semen was calculated by the angle cosine method.Results A total of 11 common peaks were calibrated in the established fingerprint.The similarity of the Gleditsiae japonica var.Delavayi Semen as a whole was lower than that of the Gleditsiae sinensis Semen samples,ranging from 0.783 to 0.842,but the similarity of the samples of the Gleditsiae sinensis Semen samples was above 0.898 except for Bozhou.The results of cluster analysis showed that 13 batches of the Gleditsiae sinensis Semen samples and 3 batches of the Gleditsiae japonica var.Delavayi Semen samples were grouped into 4 categories,and the results of principal component analysis were basically consistent with those of cluster analysis.Conclusion This method can effectively distinguish the Gleditsiae sinensis Semen and Gleditsiae japonica var.Delavayi Semen,which provides a technical guarantee for controling the quality of the Gleditsiae sinensis Semen.
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