Molecular mechanism of Duzhong-Niuxi in the treatment of osteoarthritis by regulating chondrocyte apoptosis and inflammatory response
Objective To investigate the molecular mechanism of Duzhong-Niuxi in the treatment of osteoarthritis(OA).Methods Thirty SD rats were randomly divided into blank control group,model group,low dose group of Duzhong-Niuxi,middle dose group of Duzhong-Niuxi and high dose group of Duzhong-Niuxi,with 6 rats in each group.Except for blank control group,OA models were established in the other four groups.After the model was successfully established,blank control group and model group were given normal saline by gavage,and the low,medium and high dose groups of Duzhong-Niuxi were given the corresponding volume of water decoction of Duzhong-Niuxi by gavage,once a day for 4 weeks.At the end of the treatment,abdominal aortic blood samples were collected;knee joint and cartilage tissues were collected.The morphological changes of knee joint were observed by HE staining.The expression levels of Bax and Bcl-2 were detected by RT-PCR and Western Blot,and the serum concentrations of TNF-α and IL-1 β were detected by ELISA.Results Compared with blank control group,the cartilage in model group showed obvious degeneration and the number of cells was significantly reduced.While after the administration of Duzhong-Niuxi,the cartilage was arranged more completely and the number of cells was slightly reduced.Compared with blank control group,the expression level of Bax,the serum concentrations of TNF-α and IL-1 β were increased,while the expression level of Bcl-2 was decreased in model group;the expression levels of the above genes showed opposite results in a dose-dependent manner.Conclusion Duzhong-Niuxi can delay OA by inhibiting the expression of Bax,TNF-αand IL-1 β,and promoting the expression of Bax,inhibiting the apoptosis of chondrocytes and inflammatory response.
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