Impacts of triptolide on tumor growth,immune microenvironment and TLR4/NF-κB signaling pathway in MGC-803 gastric cancer bearing mice
Objective To investigate the impacts of triptolide(TPL)on tumor growth,immune microenvironment and TLR4/NF-κB signaling pathway in MGC-803 gastric cancer bearing mice.Methods MGC-803 cells were injected subcutaneously into BALB/c male mice,and the mice were randomly divided into model group,positive drug group,TPL-L group,TPL-H group,and TPL-H+LPS group.The tumor growth and spleen and thymus indices of mice were detected;flow cytometry was applied to detect the levels of CD4+T and CD8+T in peripheral blood of mice;ELISA was applied to detect the levels of tumor necrosis factor-α(TNF-α),interleukin-2(IL-2),interferon-γ(INF-γ),and IL-4 in the peripheral blood of mice;Western Blot was applied to detect the expression of TLR4/NF-κB pathway related proteins in tumor tissues of mice.Results Compared with model group,the tumor weight and volume of gastric cancer mice in TPL group were lower,the tumor inhibition rate,spleen and thymus indexes,the levels of CD4+T,the proportion of CD4+/CD8+,the levels of TNF-α,IL-2,and INF-γ in peripheral blood were higher(P＜0.05),the levels of CD8 T and IL-4,the expression of TLR4,MyD88,TRAF6,NF-κB p65,and PD-L1 proteins in tumors was lower(P＜0.05);TLR4 activator LPS for compensatory experiments showed that LPS reversed the inhibitory effects of TPL on tumor growth and immune escape in gastric cancer mice(P＜0.05).Conclusion TPL can inhibit tumor growth,enhance cellular immune function,and inhibit the activation of TLR4/NF-κB signaling pathway in MGC-803 gastric cancer bearing mice.
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