Improving effect of Chongcao Baoshen Granules on renal osteodystrophy rats and its impact on the Klotho/FGF-23 pathway
Objective To observe the improvement effect of Chongcao Baoshen Granules(CCBSG)on renal osteodystrophy(ROD)rats and the influence on Klotho/fibroblast growth factor-23(FGF-23)pathway.Methods A total of 72 rats were randomly separated into control group,ROD group,low dose,medium dose,and high-dose CCBSG groups,and calcitriol group,12 in each group.Except control group,all other groups were used to construct ROD rat models through 5/6 nephrectomy combined with high phosphorus feeding,and after successful modeling,medication was administered once a day for 12 weeks.Automatic biochemical analyzer was applied to detect levels of urea nitrogen(BUN),creatinine(Scr),calcium(Ca2+),and phosphorus(P3+)in serum;ELISA method was applied to detect the level of intact parathyroid hormone(iPTH)in serum;dual energy X-ray was applied to detect bone mineral density of femur;HE staining was used to evaluate the pathological changes of femoral tissue;Western Blot was used to detect Klotho and FGF-23 proteins expression in femoral tissue.Results Compared with control group,the pathological damage of the femur in ROD group was severe,the levels of BUN,Scr,P3+,iPTH in serum and the expression of FGF-23 protein in femoral tissue increased,the level of Ca2+,bone density,and Klotho protein expression in femoral tissue decreased(P＜0.05);compared with ROD group,the pathological damage to the femur of rats in low dose,medium dose,and high dose CCBSG groups,and calcitriol group was alleviated,the levels of BUN,Scr,P,iPTH in serum and the expression of FGF-23 protein in femoral tissue decreased,the level of Ca2+,bone density,and the protein expression of Klotho in femoral tissue increased(P＜0.05).Conclusion The improvement of rat ROD by CCBSG may be related to the regulation of the Klotho/FGF-23 pathway.
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