基于HPLC指纹图谱不同产地味连质量评价及其细胞活力谱效关系研究
Correlation analysis between HPLC fingerprint and cell viability of Coptis chinensis
陈思妙 1简祈盼 1舒宇航 1李振辉 1王本业 1王琦 1涂济源 1周仲实 1王光忠1
作者信息
- 1. 湖北中医药大学药学院,湖北武汉 430065
- 折叠
摘要
目的 建立味连HPLC指纹图谱,研究其与细胞活力之间的谱效关系,探讨不同产地味连品质差异,明确味连寒凉之性活性成分.方法 采用HPLC法建立味连的指纹图谱并评价相似度,结合聚类分析(CA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)对其共有峰进行评价;并且以味连对Hep3B细胞的细胞活力影响为药效指标,采用灰色关联度分析(GRA)和正交偏最小二乘回归分析(PLSR)相结合分析其谱效关系.结果 建立了不同产地味连的HPLC指纹图谱,标定了 10个共有峰,全部样品的相似度均＞0.98,通过比对法归属了其中8个峰.CA结果表明,全部样品被聚为2类,与PCA结果基本一致,结合正交偏最小二乘法-判别分析(OPLS-DA)发现,小檗碱、巴马汀以及格兰地新是黄连样品组间存在差异的3个标志性化合物.不同产地的味连均有不同程度抑制细胞活力的作用,谱效关联结果表明小檗碱、巴马汀以及非洲防己碱是味连醇提物抑制Hep3B细胞活力的主要活性成分.结论 不同产地味连具有总体质量一致性与特定成分差异性的特征,小檗碱、巴马汀以及非洲防己碱是味连寒凉之性主要活性成分,为不同产地味连质量评价提供参考依据.
Abstract
Objective To establish the HPLC fingerprints of Coptis chinensis and study the relationship between its spectrum-effect and cell viability,and clarify the differences in active ingredients and quality of Coptis chinensis from different origins.Methods The fingerprints of Coptis chinensis were established by HPLC method and the similarity was evaluated by combining cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)to evaluate the shared peaks.The effect of Coptis chinensis on the cell viability of Hep3B cells was used as the efficacy index,and the spectrum-effect relationship was analyzed by a combination of grey correlation analysis(GRA)and orthogonal partial least squares regression analysis(PLSR).Results HPLC fingerprint profiles of different origins of Coptis chinensis were established,10 common peaks were identified,the similarity of all samples was greater than 0.98,and 8 peaks were attributed by comparison method.The CA results showed that all samples were clustered into 2 categories,which was in general agreement with the PCA results.Combined with orthogonal partial least squares-discriminant analysis(OPLS-DA),it was found that berberine,palmatine and grandisine were the three signature compounds responsible for the differences among the sample groups of Coptis chinensis.The spectroscopic correlations showed that berberine,palmatine,columbamine were the main components that differentiated the inhibition of cell viability in the alcoholic extracts of different origins of Coptis chinensis.Conclusion Different origins of Coptis chinensis are characterized by overall quality consistency and variability of specifiic components,and berberine,palmatine and columbamine are closely related to the inhibition of cell viability,providing a reference basis for the quality evaluation of Coptis chinensis from different origins.
关键词
味连/不同产地/指纹图谱/细胞活力/谱效关系Key words
Coptis chinensis/Different origins/Fingerprint mapping/Cell viability/Spectral-effect relationship引用本文复制引用
基金项目
湖北省中央引导地方科技发展资金项目(2020ZYYD030)
出版年
2024
NETL
NSTL
万方数据