Aerobics exercise combined with lavender essential oil effects the learning memory and inflammtory factor levels of sleep deprived rats
Objective To observe the effects of aerobic exercise combined with lavender essential oil inhalation and sniffing intervention to regulate NF-κB pathways on learning memory and inflammatory factor levels in sleep deprived rats.Methods Forty male Sprague-Dawley rats were divided rapidly into five groups:blank group,model group,exercise group,essential oil group,and exercise combined essential oil group.Exercise group performed aerobic exercise,essential oil group performed lavender essential oil inhalation and sniffing,and exercise combined essential oil group performed aerobic exercise associated with lavender essential oil inhalation and sniffing,all of which were pre-intervened for 4 weeks,and then the rats in model group,exercise group,essential oil group,and exercise combined essential oil group underwent 72 h of acute sleep deprivation.The Morris water maze was developed to test the learning and memory of rats.HE staining was used to monitor pathological morphological changes in hippocampal tissue;Western Blot was performed to detect the expression levels of IκBαand AC-NF-κB proteins in rat hippocampa;real-time quantitative fluorescence PCR and ELISA assay were used to detect the expression amounts of TNF-α,IL-6 and IL-1 β in hippocampa and serum.Results Compared with blank group,model group had considerably longer evasion latency and swimming distance,longer initial arrival time at the platform,and shorter residence time in the target quadrant(P＜0.01);rat hippocampal neurons were disorganised,with a reduced density and number;IκBαprotein expression was considerably lower,and AC-NF-κB protein expression was considerably higher(P＜0.01);and the expression levels of inflammatory factor TNF-α,IL-6,IL-1 β expression levels increase remarkably(P＜0.01).Compared with model group,rats in exercise group,essential oil group and exercise-combined essential oil group had obviously shorter latency and swimming distance,and longer residence time in the target quadrant(P＜0.05,P＜0.01);hippocampal neurons in the rats were arranged more regularly,with increased density and higher number;AC-NF-κB protein expression in exercise group was reduced,and IκBα protein expression in essential oil group and exercise-combined essential oil group was obviously increased,while AC-NF-κBα protein expression in the hippocampus and serum was obviously increased(P＜0.05),while AC-NF-κB protein expression was decreased remarkably(P＜0.05,P＜0.01);the expression of inflammatory factors TNF-α,IL-6,and IL-iβ in the hippocampus and serum was decreased remarkably(P＜0.01).Conclusion Aerobic exercise combined with lavender essential oil can attenuate the inflammatory response in the hippocampus and serum of rats,and ameliorate the learning and memory deficits induced by sleep deprivation.
Aerobic exerciseLavender essential oilSleep deprivationLearning and memoryInflammatory factors
NETL
NSTL
万方数据
