Enzymatic(4+2)-and(2+2)-cycloaddition reactions:fundamentals and applications of regio-and stereoselectivity
The(4+2)-and(2+2)-cycloadditions are important chemical reactions for constructing ring structures,with broad applications in the chemical synthesis and biosynthesis of complex natural products and chiral drugs.The discovery and development of enzymatic cycloaddition reactions,including both(4+2)-and(2+2)-cycloadditions,are currently hot topics in the field of chemical biology.Recently,several international and domestic research groups have successively reported multiple enzymatic(4+2)-and(2+2)-cycloadditions,revealing related protein structures and enzymatic mechanisms,designing new artificial cyclases,and developed different types of regio-and selective cycloaddition reactions through protein engineering.These studies provide a theoretical basis and successful examples for the design and optimization of novel cyclases using synthetic biology strategy,and will promote applications of the enzymatic reactions in organic synthesis.
(4+2)-cycloaddition reactions(2+2)-cycloaddition reactionschemical synthesis and biosynthesiscyclasesdirected evolution
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