香豆素类光敏基团对脱氧核苷5'-羟基的选择性保护
Selective Protection of 5'-Hydroxyl Group of Deoxynucleoside by Photosensitive Groups of Coumarin
董连强 1马星光 2孙亚伟1
作者信息
- 1. 中国石油大学(华东)化学化工学院,山东青岛 266580
- 2. 烟台东化新材料有限公司合成研发部,山东烟台 265500
- 折叠
摘要
以香豆素类化合物作为光可降解保护基,探究了香豆素类化合物对脱氧核苷中5'-位羟基的保护和脱保护性能,为在传统核酸合成中使用光脱保护替代酸脱保护做了前期工作.选择可在紫外及蓝光波段区间进行光化学反应的7-二乙胺基4-羟甲基香豆素类化合物作为保护基团,并以碳酸酯的形式连接在N-4-乙酰基脱氧胞苷的5'-羟基上,考察该化合物在紫外和蓝光光照下的光化学性质,为构建光调节DNA合成进行前期研究并奠定良好基础.
Abstract
In this paper,we utilized the photolabile coumarin compounds to replaced the acid remova-ble protective groups on the 5'-hydroxyl group on the deoxyriboside in nucleic acids'synthesis.The violent and blue light responsive coumarin segments were attached on the 5'-hydroxyl group on the N-4 acetyl deoxycytidine via the carbonate linker,and the synthesis photolysis was investigated under dif-ferent light irradiation.
关键词
光可降解/香豆素/核苷/可见光响应/光敏单元合成/DNA合成Key words
photalabile/coumarin/deoxynucleoside/visible light responsive/photosensitive unit syn-thesis/DNA synthesis引用本文复制引用
出版年
2024
NETL
NSTL
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