首页|一种极性依赖性双光子溶酶体荧光探针的合成及性能

一种极性依赖性双光子溶酶体荧光探针的合成及性能

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探针Lyso-LCL是一种极性依赖双光子荧光探针,具有低毒性,可成功靶向溶酶体.以 4-溴-1,8-萘二甲酸酐为原料合成了具有D-π-A结构的荧光探针Lyso-LCL,通过1 H NMR,13 C NMR和HR-MS(ESI)对目标分子结构进行了表征,并对Lyso-LCL的紫外吸收光谱、荧光发射光谱以及双光子荧光光谱进行了测试和分析.结果表明:该探针具有溶剂致变色效应,随着溶剂极性的增大,探针的荧光发射峰发生明显红移,且荧光强度下降,并且探针具有双光子性质.此外,探针Lyso-LCL具有低毒性,可成功靶向溶酶体,与溶酶体商品化染料的共定位系数高达0.93.因此,Lyso-LCL可作为一种新型的极性荧光探针,用于细胞溶酶体靶向成像.
Synthesis and Properties of A Lysosomal Polarity-specific Two-photo Fluorescent Probe
The probe Lyso-LCL is a polar-dependent two-photon fluorescence probe with low toxicity that can successfully target lysosomes.A novel donor-π-acceptor(D-π-A)type of fluorescent probe,Lyso-LCL,based on the 4-bromo-1,8-naphthalic anhydride has been developed.The structures of these compounds were assigned based on the results from 1 H NMR,13 C NMR spectroscopy and HR-MS(ESI).Moreover,the optical properties of Lyso-LCL,such as UV absorption spectrum and fluores-cence emission spectrum,were further investigated.The results show that it has a significant response to the polarity with a solvatochromic effect.With the increase in solvent polarity,the fluorescence e-mission peak of Lyso-LCL is red-shifted and the fluorescence intensity is decreased.Importantly,Lyso-LCL had the large two-photon absorption cross-section and good two-photon absorption perform-ance.Moveover,Lyso-LCL has the low cytotoxicity and accurate lysosome-targeting ability,and the pearson correlation coefficient is 0.93.Therefore,Lyso-LCL could be used as a novel polarity-specif-ic fluorescent probe and lysosomal tracking agent for cell imaging.

D-π-Afluorescent probesolvatochromic effecttwo-photonpolaritylysosome-targeting

王碧玉、刘成露、徐奥琼、冯可心、李龙春

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安徽农业大学 理学院,安徽 合肥 230036

D-π-A 荧光探针 溶致变色 双光子 极性 溶酶体靶向

安徽省大学生创业训练计划项目安徽农业大学大学生创业训练计划项目2020年度安徽省自然科学基金资助项目

S2021103640922021103641942008085MB41

2024

合成化学
四川省化学化工学会 中国科学院成都有机化学研究所

合成化学

CSTPCD
影响因子:0.42
ISSN:1005-1511
年,卷(期):2024.32(2)
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