The carboxyl group of humus derivatives can acylation or diazotization with protein mole-cules,which can quickly analyze and detect pesticide residues,and has potential application value in the field of food safety.Using methyl 2-chloropropionate and methyl methacrylate as raw materials,the humus hapten derivatives were synthesized through an 8-step reaction of nucleophilic substitution,ester hydrolysis,dehydration,alkylation,nitro reduction,amino nucleophilic substitution,acid amine con-densation and trifluoroacetic acid hydrolysis.Their structures were characterized by 1H NMR,13C NMR and MS(ESI).The key synthesis process was optimized.The results showed that the reac-tion time of methyl 2-chloropropionate and methyl methacrylate was 96 h.The yield of the nucleophilic substitution reaction was up to 70％.In the nitro reduction process,when dichlorostannous hydrate was used as the reducing agent and the ratio of the amount of dichlorostannous hydrate to the amount of intermediate 8 was 4.0∶1.0,the nitro reduction reaction yield was the highest 63％.The maximum yield of aminoacylation was 50％when the ratio of compound 5 to compound 9 was 1.5∶1.0 and the reaction temperature was 85 ℃.
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