Synthesis,AIE Properties and Cell Imaging of Novel Tetraphenylethylene-based Flavonoid
Tetraphenylene(TPE)is a typical aggregation-induced emission(AIE)molecule.In this paper,a novel flavonoid(TPF)was synthesized and characterized based on TPE scaffold using 1,3-benzenediol as starting material.The spectroscopic properties of TPF in different solvents and mixed solvents were investigated,and the results showed that the fluorescence intensity(FI)of TPF in mixed solvents of THF and water increased with the increase of volumetric water content,and FI was the strongest in 70％volumetric water content.Based on theoretical calculations,it was found that TPF had propeller configuration,and the aggregation of molecules in poor solvents led to the restriction of the movement and thus led to the AIE phenomenon.In vitro cytotoxicity tests showed that TPF had low toxicity against HepG2,and the cell survival rate was higher than 85％at a concentration of 40 μM.In addition,TPF was able to enter HepG2 cells and be used for fluorescence imaging in living cells.Therefore,TPF can be used as a reporter for the design of fluorescent probes.
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