Secondary structures of protein amideⅢ band and its thermal denaturation in natural rub-ber were studied by midinfrared(MIR)spectroscopy.The result of variable temperaturet deconvolution MIR spectroscopy showed that the infrared absorp-tion peak intensity of irregular curled,β-folded,α-spiral and β-corner se-condary structures decreased when the temperature increased from 293 to 393 K.The result of two-dimensional MIR spectroscopy showed that there were significant differences in the sensitivity of amide Ⅲ band secondary structures to heat,with the irregular curled secondary structure being the first to change.Under thermal disturbance factor,the irregular curled secondary structure change was a key step in the overall secondary structure change of the amide Ⅲ band.
关键词
天然橡胶/蛋白质二级结构/酰胺Ⅲ带/二维中红外光谱/热变性
Key words
natural rubber/secondary structure of protein/amide Ⅲ band/two-dimensional mid-in-frared spectroscopy/thermal denaturation
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