摘要
结合层次分析法(AHP)-熵权法(EWM),通过单因素实验考察功率、液固比、提取时间、提取次数和粒度对黄芩苷、汉黄芩苷、黄芩素和汉黄芩素得率及出膏率的影响.以综合评分为响应值,利用四因素三水平Box-Behnken(BBD)响应面法优化黄芩的微波提取工艺,并通过扫描电子显微镜(SEM)观察提取前后药材粉末的微观形态变化.BBD响应面法得到的最优提取工艺条件为:微波功率 390 W、液固比 56 mL/g、提取时间 472 s、粒度 80 目(180 μm),此时黄芩苷、汉黄芩苷、黄芩素、汉黄芩素得率分别为 16.82%、3.46%、0.30%、0.31%,出膏率 73.73%,综合评分 10.75.该工艺提取效果优于常见的回流提取方法,实现评价结果主客观的内在统一.
Abstract
Combined with analytic hierarchy process-entropy weight method,the effects of microwave power,liquid-solid ratio,extraction time,extractions times and particle size were investigated in single factor test using the yield of baicalin,wogonoside,baicalein,wogonin and paste yield as the indexes.Taking the comprehensive score as the response value,the microwave extraction process of Scutellaria baicalensis was optimized by the Box-Behnken response surface test at four factors and three levels.The micro-morphological changes of medicinal powder before and after extraction were observed by SEM.The results showed that the optimal extraction process conditions obtained by BBD response surface method were microwave power of 390 W,liquid-solid ratio of 56 mL/g,extraction time of 472 s,and particle size of 80 mesh(180 μm).At this time,the yield of baicalin,wogonoside,baicalein and wogonin were 16.82%,3.46%,0.30%and 0.31%,respectively,the paste yield was 73.73%,and the comprehensive score was 10.75.The extraction effect of this process is better than that of common reflux extraction,and the subjectivity and objectivity of the evaluation results are realized.
维普
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