Circulating CCRR serves as potential novel biomarker for predicting acute myocardial infarction

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外文摘要：Objective:Cold regions exhibit a high prevalence of cardiovascular disease,particularly acute myocardial infarction(AMI),which is one of the leading causes of death associated with cardiovascular conditions.Cardiovascular disease is closely linked to the abnormal expression of long non-coding RNA(lncRNA).This study investigates whether circulating levels of lncRNA cardiac conduction regulatory RNA(CCRR)could serve as a biomarker for AMI.Materials and methods:We measured circulating CCRR from whole blood samples collected from 68 AMI patients and 69 non-AMI subjects.An AMI model was established using C57BL/6 mice.Quantitative reverse transcription PCR(qRT-PCR)was used to assess CCRR expression.Exosomes were isolated from cardiomyocytes,and their characteristics were evaluated using electron microscope and nanoparticle tracking analysis.The exosome inhibitor GW4869 was employed to examine the effect of exosomal CCRR on cardiac function using echocardiography.Protein expression was detected using Western blot and immunofluorescence staining.Results:The circulating level of CCRR was significantly higher in AMI patients(1.93±0.13)than in non-AMI subjects(1.00±0.05,P<0.001).The area under the ROC curve(AUC)of circulating CCRR was 0.821.Similar changes in circulating CCRR levels were consistently observed in an AMI mouse model.Exosomal CCRR derived from hypoxia-induced cardiomyocytes and cardiac tissue after AMI were increased,a change that was reversed by GW4869.Additionally,CCRR-overexpressing exosomes improved cardiac function in AMI.Conclusion:Circulating lncRNA CCRR is a potential predictor of AMI.Exosomal CCRR plays a role in the communication between the heart and other organs through circulation.

外文关键词：

acute myocardial infarctionlncRNAcardiac conduction regulatory RNAexosome

作者：

Lina Xuan、Huishan Luo、Shu Wang、Guangze Wang、Xingmei Yang、Jun Chen、Jianjun Guo、Xiaomeng Duan、Xiufang Li、Hua Yang、Shengjie Wang、Hailong Zhang、Qingqing Zhang、Shulei Liu、Yongtao She、Kai Kang、Lihua Sun

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作者单位：

Department of Pharmacology,Harbin Medical University(the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China,Key Laboratory of Cardiovascular Research,Ministry of Education,Joint International Research Laboratory of Cardiovascular Medicine Research,Ministry of Education),College of Pharmacy,State Key Laboratory of Frigid Zone Cardiovascular Diseases(SKLFZCD),Harbin Medical University,Harbin 150000,China

Department of Cardiology,the First Affiliated Hospital,Harbin Medical University,Harbin 150000,China

Department of Cardiovascular Surgery,the First Affiliated Hospital of Harbin Medical University,Harbin 150000,China

NHC Key Laboratory of Cell Transplantation,the First Affiliated Hospital of Harbin Medical University,Harbin 150000,China

Department of Cardiovascular Surgery,the First Affiliated Hospital of Harbin Medical Un

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基金：

Natural science Foundation of ChinaNatural science Foundation of ChinaNatural science Foundation of Heilongjiang Province,ChinaOutstanding Young Talent Research Fund of College of Pharmacy,Harbin Medical University2021(the second batch)Research Funds for affiliated research institutes in Heilongjiang Province

项目编号：

8197020281903609LH2022H0022019-JQ-02CZKYF2021-2-C013

出版年：

2024

DOI：

10.1515/fzm-2024-0015

寒地医学(英文)

黑龙江省卫生健康发展研究中心

寒地医学(英文)

ISSN：2096-9074

年,卷(期)：2024.4(3)