Establishment of a method forβ-sitosterol determination in Xikangalmond
The phytosterol content in Xikang almond kernels is rich and dominated by β-sitosterol.By ex-ploring the conditions such as wavelength,mobile phase ratio,flow rate and column temperature detected by high-performance liquid chromatography,the content of β-sitosterol in Xikang almond kernel was deter-mined.The optimal conditions for the determination of β-sitosterol content are detection wavelength of 201 nm,mobile phase of 100%methanol,flow rate of 1.0 mL/min and chromatographic column temperature of 40℃.The methodological investigation results indicate that the method has a good linear relationship with RSD values of precision repeatability and stability are less than 2.0%,and an average spiked recov-ery rate is 106.14%.The method is stable and reliable which can be used for the content determination of β-sitosterol in Xikang almond kernel.
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