西康扁桃仁中β-谷甾醇检测方法的建立
Establishment of a method forβ-sitosterol determination in Xikangalmond
朱子冬 1李仁爱 1鲁泽立 1程潇潇 1田荣荣1
作者信息
- 1. 北京城市学院生物医药学部,北京 100094
- 折叠
摘要
西康扁桃仁中植物甾醇含量丰富,且以β-谷甾醇为主.通过高效液相色谱法检测波长、流动相配比、流速、柱温等条件,确定西康扁桃仁中β-谷甾醇含量测定的最佳条件为检测波长201 nm,流动相为 100%甲醇,流速 1.0 mL/min,色谱柱温度40℃.方法学考察结果表明,该方法线性关系良好,精密度、重复性及稳定性的RSD值均小于2.0%,平均加标回收率 106.14%,该方法稳定可靠,可用于西康扁桃仁中 β-谷甾醇的含量测定.
Abstract
The phytosterol content in Xikang almond kernels is rich and dominated by β-sitosterol.By ex-ploring the conditions such as wavelength,mobile phase ratio,flow rate and column temperature detected by high-performance liquid chromatography,the content of β-sitosterol in Xikang almond kernel was deter-mined.The optimal conditions for the determination of β-sitosterol content are detection wavelength of 201 nm,mobile phase of 100%methanol,flow rate of 1.0 mL/min and chromatographic column temperature of 40℃.The methodological investigation results indicate that the method has a good linear relationship with RSD values of precision repeatability and stability are less than 2.0%,and an average spiked recov-ery rate is 106.14%.The method is stable and reliable which can be used for the content determination of β-sitosterol in Xikang almond kernel.
关键词
西康扁桃仁/β-谷甾醇/高效液相色谱法/含量测定Key words
Xikang almond/β-sitosterol/high performance liquid chromatography/content determination引用本文复制引用
基金项目
北京城市学院大学生创新创业项目(202211418019)
出版年
2024
NETL
NSTL
万方数据