In order to improve the extraction and separation efficiency of 5-hydroxy-L-tryptophan(5-HTP)and reduce energy consumption and pollution emission,ceramic ultrafiltration membrane was selected to extract 5-HTP from fermentation broth.Firstly,the suitable membrane pore size of 10 nm was selected,and then the three optimum process parameters for the separation and extraction of 5-HTP by ceramic ultrafiltration membrane were further optimized:pH value of fermentation solution is 3.5,transmembrane pressure is 0.75 MPa,and transmembrane temperature is 35 ℃.Under this process condition,the penetration ratio of 5-HTP is more than 92％,and the rejection ratio of free protein is more than 90％,which meets the requirements of further concentration and crystallization to prepare 5-HTP,and achieves the expected effect of the optimization.It provides a reliable basis for the application of ceramic ultrafiltration membrane to industrial extraction of 5-HTP from fermentation broth.
关键词
陶瓷超滤膜/5-羟基-L-色氨酸/过滤条件/透过率/截留率
Key words
ceramic ultrafiltration membrane/5-hydroxy-L-tryptophan/filtration condition/permeate ratio/rejection ratio
维普
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