首页|利用生物信息学筛选动脉粥样硬化脂质代谢基因

利用生物信息学筛选动脉粥样硬化脂质代谢基因

扫码查看
为挖掘动脉粥样硬化脂质代谢相关基因,从基因表达综合(gene expression omnibus,GEO)数据库中下载数据集,利用加权相关网络分析(weighted correlation network analysis,WGCNA)、差异分析以及韦恩图筛选关键基因,并进行疾病相关性分析.结果显示,10个关键基因HMGCR、HMGCS1、LDLR、DHCR24、DHCR7、SQLE、IDI1、INSIG1、FDFT1、MSMO1 与低密度脂蛋白受体(low density lipoprotein receptor,LDLR)呈正相关;HMGCR、DHCR7 和 INSIG1 与白介素 10(interleukin-10,IL10)呈正相关;除DHCR7 外,其余 9 个基因与 ATP 结合盒转运体 A1(ATP-binding cassette transporter A1,ABCA1)呈负相关;DHCR24、SQLE、IDI1、INSIG1 和 FDFT1 与过氧化物酶体增殖物激活受体 γ(peroxisome proliferator activated receptor gamma,PPARG)呈负相关,差异具有统计学意义(P<0.05).GO和KEGG富集分析显示,关键模块基因主要富集于类固醇、胆固醇和甾醇生物合成及代谢过程.10个关键基因可为动脉粥样硬化有效治疗靶点的筛选与研发提供借鉴作用.
Bioinformatics analysis of the genes related to lipid metabolism in atherosclerosis
To explore the genes related to lipid metabolism in atherosclerosis,the dataset was downloaded from the gene expression omnibus(GEO)database,and weighted correlation network analysis(WGCNA),differential analysis and Venn diagram were used to screen the key genes,and then the disease correlation analysis was carried out.The results showed that,the ten key genes of HMGCR,HMGCS1,LDLR,DHCR24,DHCR7,SQLE,IDI1,INSIG1,FDFT1,MSMO1 were positively correlated with low density lipoprotein receptor(LDLR);and HMGCR,DHCR7 and INSIGI were positively correlated with interleukin-10(IL10);and except DHCR7,the other nine genes were negatively correlated with ATP-binding cassette transporter A1(ABCA1);and DHCR24,SQLE,IDI1,INSIG1 and FDFT1 were negatively correlated with peroxisome proliferator activated receptor gamma(PPARG),the difference was statistically significant(P<0.05).GO and KEGG enrichment analysis showed that,the key module genes were mainly enriched in steroid,cholesterol and sterol biosynthesis and metabolism processes.The screening of key genes can provide reference for the screening and development of effective therapeutic targets for atherosclerosis.

atherosclerosisbioinformaticslipid metabolismenrichment analysisdisease correlation analysis

申俊敏、林芳蕊、王宝沣、刘莉

展开 >

河北大学基础医学院,河北保定 071000

动脉粥样硬化 生物信息学 脂质代谢 富集分析 疾病相关性分析

河北大学多学科交叉项目中央引导地方科技发展资金项目河北省省级研究生示范课程建设项目

2021DXKJC13226Z2604GKCJSX2022010

2024

河北大学学报(自然科学版)
河北大学

河北大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.322
ISSN:1000-1565
年,卷(期):2024.44(2)
  • 29