Evaluation the iron supplementation effect of glutamate chelated iron on iron deficiency anemia of mice
Glutamate chelated ferric iron(Glu-Fe(Ⅲ))and glutamate chelated ferrous iron(Glu-Fe(Ⅱ))were prepared and their iron supplementation effects were evaluated.The iron deficiency anemia(IDA)mouse model was established by combining low iron feed and bloodletting.IDA mice were randomly divided into five groups(n=5):ferrous sulfate group,commercially available iron dextran group,Glu-Fe(Ⅲ)group,Glu-Fe(Ⅱ)group and negative controls group,using normal mice as blank controls.Different iron supplements at equal iron doses were given by intragastric administration for 4 weeks.The changes of hemoglobin(HGB),red blood cells(RBC)and hematocrit(HCT)in blood were monitored by automatic hematology analyzer.The iron status of IDA mice were determined by serum iron(SI),total iron binding capacity(TIBC)and transferrin(TRF)kits and the iron contents in mouse tissues(heart,liver,spleen and kidney)were measured.Superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)kits were used to detect the effects of various iron supplements on antioxidant activity of IDA mice in vivo.The results showed that IDA mouse model was successfully established.Glu-Fe(Ⅲ)and Glu-Fe(Ⅱ)could improve the HGB,RBC and HCT values of IDA mice,meanwhile,reduce TIBC and TRF content and increase SI level for administrated IDA mice,especially Glu-Fe(Ⅲ).Compared with ferrous sulfate and iron dextran,Glu-Fe(Ⅲ)was more effective in reducing the swollen of organs(heart,spleen and kidney),improving liver recovery,enhancing iron storage in liver and spleen,scavenging tissue ROS and enhancing antioxidant activity in IDA mice.
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