Inhibition of baicalin on macrophage inflammasome activation induced by Listeria monocytogenes
In order to study the effect of baicalin on the inflammasome of mouse macrophages activated by Listeria monocytogenes(LM),the optimal concentration gradient of baicalin was determined by methylthiazolyldiphenyl-tetrazolium bromide(MTT)method.Then macrophages were treated with LM and baicalin at concentrations of 25,50 and 100 μg/mL,respectively,to detect the expression level of inflammasome-related protein or mRNA and lactate dehydrogenase(LDH)release.The results showed that baicalin reduced IL-1β,caspase-1 p10 protein levels,LDH release and mRNA levels of NLRP3,NLRC4,NLRP10,NOD,AIM2,caspase-1,IL-1β and IL-18 after LM treatment in a dose-dependent manner.Therefore,it is speculated that baicalin can inhibit the release of inflammatory factors and cell death in macrophages by inhibiting the activation of different inflammasome in macrophages induced by LM.
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