4种夏栎对低温胁迫的生理响应及抗寒性评价
Physiological response and cold resistance evaluation of four species of Quercus robur to low temperature stress
姜恒 1张志刚 2杨建军 1李斌 3王兴胜 3张晓红3
作者信息
- 1. 新疆大学生态与环境学院,绿洲生态教育部重点实验室,新疆乌鲁木齐 830017;自然资源部荒漠-绿洲生态监测与修复工程技术创新中心,新疆乌鲁木齐 830002
- 2. 新疆林业科学院造林治沙研究所,新疆林木资源与利用国家林草局重点实验室,新疆乌鲁木齐 830000
- 3. 伊犁州林木良种繁育试验中心,新疆察布查尔 835311
- 折叠
摘要
为探究低温胁迫下夏栎(Quercus robur)生理指标的响应及抗寒性,以普通夏栎、速生夏栎、裂叶夏栎和垂枝夏栎为实验材料,测定在不同温度下细胞膜通透性、超氧化物歧化酶(SOD)、过氧化物酶(POD)、可溶性蛋白等指标,并利用拟合Logistic方程计算半致死温度,结合隶属函数法对4种夏栎综合评价.结果表明:4种夏栎细胞膜通透性均随着温度降低而增加,SOD和POD活性以及可溶性蛋白、脯氨酸和丙二醛(MDA)含量均逐渐下降;4种夏栎半致死温度为一41.9～-38.2 ℃,其中普通夏栎半致死温度最低,速生夏栎半致死温度最高;4种夏栎抗寒性强弱为普通夏栎＞垂枝夏栎＞裂叶夏栎＞速生夏栎.综上可知,普通夏栎抗寒性最强,在不同地区推广和繁育具有显著优势.
Abstract
In order to explore the response of Quercus robur physiological indicators and the strength of cold resistance under low temperature stress,the common Q.robur,fast-growing Q.robur,split-leaf Q.robur and weeping Q.robur were used as experimental materials.The cell membrane permeability,SOD activity,POD activity and soluble protein at different temperatures were measured,and the fitted Logistic equation was used to calculate the half-lethal temperature.And the function method was used for comprehensive evaluation of the four Q.robur species.The results showed that the cell membrane permeability of the four species of Q.robur showed an increasing trend as the temperature decreased,and the SOD activity,POD activity,soluble protein content,proline content and MDA content showed a decreasing trend as a whole,and the cell membrane permeability was calculated by combining the Logistic equation.The semi-lethal temperature of four species of Q.robur ranged from-41.9 ℃ to-38.2 ℃.The common Q.robur had the highest semi-lethal temperature and the fast-growing Q.robur had the lowest semi-lethal temperature.This is consistent with the evaluation by the membership function method.The four species of Q.robur had strong cold resistance.The cold resistance ranking of the four Q.robur species was common Q.robur＞weeping Q.robur＞split-leaf Q.robur＞fast growing Q.robur.The study show that common Q.robur is the most resistant to cold and has significant advantages for promotion and breeding in different regions.
关键词
夏栎/抗寒性/隶属函数法/生理指标/半致死温度Key words
Quercus robur/cold resistance/membership function method/physiological index/half-lethal temperature引用本文复制引用
基金项目
新疆三农骨干人才培养项目(2022SNGGNT082)
新疆维吾尔自治区2023年度重大科技专项(2023A02008)
出版年
2024
NETL
NSTL
万方数据