In order to explore the response of Quercus robur physiological indicators and the strength of cold resistance under low temperature stress,the common Q.robur,fast-growing Q.robur,split-leaf Q.robur and weeping Q.robur were used as experimental materials.The cell membrane permeability,SOD activity,POD activity and soluble protein at different temperatures were measured,and the fitted Logistic equation was used to calculate the half-lethal temperature.And the function method was used for comprehensive evaluation of the four Q.robur species.The results showed that the cell membrane permeability of the four species of Q.robur showed an increasing trend as the temperature decreased,and the SOD activity,POD activity,soluble protein content,proline content and MDA content showed a decreasing trend as a whole,and the cell membrane permeability was calculated by combining the Logistic equation.The semi-lethal temperature of four species of Q.robur ranged from-41.9 ℃ to-38.2 ℃.The common Q.robur had the highest semi-lethal temperature and the fast-growing Q.robur had the lowest semi-lethal temperature.This is consistent with the evaluation by the membership function method.The four species of Q.robur had strong cold resistance.The cold resistance ranking of the four Q.robur species was common Q.robur>weeping Q.robur>split-leaf Q.robur>fast growing Q.robur.The study show that common Q.robur is the most resistant to cold and has significant advantages for promotion and breeding in different regions.
Quercus roburcold resistancemembership function methodphysiological indexhalf-lethal temperature