In order to establish detection of lorazepam in milk,high performance liquid chromatogra-phy(HPLC)method was used,The conditims are:dissolved in 0.2％formic acid,centrifugal extraction of lorazepam residual in acetonitrile solution,repeat 3 times,C18 solid phase extraction column,eluent af-ter nitrogen blow dry with volume fraction of 50％methanol aqueous solution dissolve,0.22 mu m filter membrane filtration.An Agilent ZORBAX SB-C18 column(4.6 mm×150 mm,5 μm)was used as the an-alytical column with methanol:water(V/V)=68∶32 as the mobile phase.The detection was carried out at 230 nm with a UV detector.The results showed that:the linear range of lorazepam was 40～500 μg/mL,the regression equation was y=4.047x-36.11,the correlation coefficient was 0.999,the limit of detection was 0.927 μg/mL,the limit of quantification was 3.097 μg/mL,and the additive levels in milk were 70,180,300 μg/mL,respectively.The recoveries ranged from 81.27％to 97.58％with relative standard deviations(RSD)of 1.1％,intra-day and inter-day RSD of 1.2％to 2.6％.The method is sensi-tive,accurate,simple and quick,and suitable for the determination and analysis of lorazepam in milk.
关键词
劳拉西泮/牛奶/高效液相色谱法/外标法
Key words
lorazepam/milk/high performance liquid chromatography/external standard method
维普
万方数据