In order to make full use of seawater resources and continuously improve the economic and ecological benefits of aquaculture,the experiment of multi-trophic level comprehensive aquaculture of Penaeus chinensis intercropped with Takif ugu rubripes or the hybrid grouper Epinephelus Lanceo-latus♂ × E.f usco guttatus♀ was carried out.P.chinensis was stocked at 120,000 ind./hm2.T.ru-bripes was stocked at 490~667 ind./hm2 with the size of 230 g/ind.,the hybrid grouper was stocked at 851 ind./hm2 with the size of 25 g/ind.,612 ind./hm2 and 800 ind./h m2 with the size of 170 g/ind.,respectively.After 5 months of culture,in the polyculture of P.chinensis and T.ru-bripes,the yield of P.chinensis and T.rubripes was 3 040.5 kg/hm2 and 546.9 kg/hm2,respectively.In the polyculture of P.chinensis and the hybrid grouper,the yield of P.chinensis and the hybrid grouper was 3 474 kg/hm2 and 308.8 kg/hm2,respectively with the stocking size of the hybrid grouper 170 g/ind.,the yield of P.chinensis and the hybrid grouper was 3 562.5 kg/hm2 and 180.2 kg/hm2,respectively with the stocking size of the hybrid grouper 25 g/ind.