Effect of restrictive amino acid supplementation in fishmeal-free diets on antioxidant and some immune enzyme activities in common carp(Cyprinus carpio)
In order to investigate the impact of restrictive amino acid supplementation in fishmeal-free diets on the antioxidant properties and immune enzyme activity of common carp(Cyprinus carpio),five groups of isonitrogenous and isolipid feeds were prepared,with varying levels of restrictive amino acid(Met:Lys=0.318)supplementation:0%(group G1),0.5%(group G2),1.0%(group G3),1.5%(group G4),and 2.0%(group G5).In total,150 fish with an initial body weight of(55.54±0.09)g were selected for an 8-week culture trial.The results indicated that fishmeal-free diets supplemented with appropriate amounts of restrictive amino acids increased the total antioxidant capacity(T-AOC),total superoxide dismutase(T-SOD),and catalase(CAT)ac-tivities,while decreasing the malondialdehyde(MDA)content in the carp.CAT activity was signifi-cantly higher in groups G2,G3,G4,and G5 compared to the control group(P<0.05),and the MDA content was significantly lower in group G5 compared to groups G1,G2,and G3(P<0.05).The ac-tivity of acid phosphatase(ACP),alkaline phosphatase(AKP),and lysozyme(LZM)in the carp liver increased with higher levels of restrictive amino acid supplementation.The ACP activity in groups G3,G4,and G5 was significantly higher than that in the control group(P<0.05),and the AKP and LZM activities in groups G4 and G5 were significantly higher than those in the control group(P<0.05).In conclusion,under the experimental conditions of this study,fishmeal-free diets supple-mented with appropriate amounts of restrictive amino acids improved the antioxidant performance and some immune enzyme activities in common carp,with the best results observed with 2.0%restrictive amino acid supplementation.
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