摘要
为探讨在无鱼粉饲料中补充限制性氨基酸对鲤(Cyprinus carpio)抗氧化性能和免疫酶活性的影响,研究设计了 5 组等氮等脂的饲料,分别添加不同水平的限制性氨基酸(Met∶Lys=0.318),即 0%(G1 组)、0.5%(G2 组)、1.0%(G3 组)、1.5%(G4 组)、2.0%(G5 组).选取初始体质量为(55.54±0.09)g 的鲤 150尾,进行了为期 8 周的养殖试验.研究结果显示,适量补充限制性氨基酸能够提高鲤的总抗氧化能力(T-AOC)、总超氧物歧化酶(T-SOD)和过氧化氢酶(CAT)活性;同时降低丙二醛(MDA)含量.G4 和 G5 组的T-AOC活性显著高于对照组(P<0.05),G2、G3、G4 和G5 组的T-SOD和CAT活性显著高于对照组(P<0.05),G5 组的 MDA含量显著低于G1、G2 和G3 组(P<0.05).随着限制性氨基酸补充水平的增加,鲤肝脏的 ACP、AKP和 LZM活性也呈上升趋势.G3、G4 和 G5 组的 ACP 活性显著高于对照组(P<0.05),G4 和G5 组的 AKP和 LZM活性显著高于对照组(P<0.05).根据试验结果,可以得出结论:在本试验条件下,适量补充限制性氨基酸能够增强鲤的抗氧化性能和部分免疫酶活性,其中 2.0%的限制性氨基酸补充量效果最好.
Abstract
In order to investigate the impact of restrictive amino acid supplementation in fishmeal-free diets on the antioxidant properties and immune enzyme activity of common carp(Cyprinus carpio),five groups of isonitrogenous and isolipid feeds were prepared,with varying levels of restrictive amino acid(Met:Lys=0.318)supplementation:0%(group G1),0.5%(group G2),1.0%(group G3),1.5%(group G4),and 2.0%(group G5).In total,150 fish with an initial body weight of(55.54±0.09)g were selected for an 8-week culture trial.The results indicated that fishmeal-free diets supplemented with appropriate amounts of restrictive amino acids increased the total antioxidant capacity(T-AOC),total superoxide dismutase(T-SOD),and catalase(CAT)ac-tivities,while decreasing the malondialdehyde(MDA)content in the carp.CAT activity was signifi-cantly higher in groups G2,G3,G4,and G5 compared to the control group(P<0.05),and the MDA content was significantly lower in group G5 compared to groups G1,G2,and G3(P<0.05).The ac-tivity of acid phosphatase(ACP),alkaline phosphatase(AKP),and lysozyme(LZM)in the carp liver increased with higher levels of restrictive amino acid supplementation.The ACP activity in groups G3,G4,and G5 was significantly higher than that in the control group(P<0.05),and the AKP and LZM activities in groups G4 and G5 were significantly higher than those in the control group(P<0.05).In conclusion,under the experimental conditions of this study,fishmeal-free diets supple-mented with appropriate amounts of restrictive amino acids improved the antioxidant performance and some immune enzyme activities in common carp,with the best results observed with 2.0%restrictive amino acid supplementation.
基金项目
辽宁省教育厅青年科技人才"育苗"项目(QL202004)
大连海洋大学"蔚蓝英才"人才计划()
NETL
NSTL
万方数据