Transcriptomic analysis of the impact of artificial compound feed cultivation on gene expression in the intestinal tissue of Mandarin fish
To investigate the adaptability of mandarin fish(Siniperca chuatsi)intestinal tissue to feed intake,two groups of fish were fed with feed for 30 days.The control group had not been fed with ar-tificial feed before.The experimental group of fish had been tamed to feed.Utilizing RNA-seq tech-nology,transcriptomic data from the intestinal tissues of mandarin fish were analyzed.The RNA-seq sequencing yielded a total of 41.65 Gb of clean data,with a Q30 base percentage exceeding 90%.Transcriptome sequencing analysis identified 955 differentially expressed genes under the screening criteria of FDR≤0.05 and ︱log2 FoldChange︱≥1.GO functional enrichment analysis revealed sig-nificant enrichment of differentially expressed genes primarily associated with organic acid metabolism processes.Additionally,KEGG pathway enrichment analysis demonstrated significant enrichment of differential genes in pathways such as the cell cycle and the PPAR signaling pathway.In summary,to adapt to aquaculture feeding,mandarin fish activated the expression of genes related to dietary signa-ling pathways,thereby enhancing the digestive and absorptive capacities of intestinal tissue for artifi-cial compound feeds.
万方数据
维普
