Primer design and evaluation for quantitative PCR analysis of growth hormone geneexpression in Clarias gariepinus
This experiment focused on the design and evaluation of primers for growth hormone(GH)gene in Clarias gariepinus using fluorescence quantitative PCR(qPCR).Five pairs of primers were synthesized,and the GHF-GHR primer pair was selected based on the results of conventional PCR electrophoresis.Subsequently,the GHF-GHR primer pair was evaluated for qPCR.The results in-dicated that the amplification curve of the GH gene fragment with the GHF-GHR primer pair showed a single peak,demonstrating good specificity of the primers.The amplification efficiency of the GHF-GHR primer pair was determined to be 93.3%based on the standard curve,with an R2 value of 0.982,indicating a high amplification efficiency of the primers.These results suggest that the GHF-GHR primer pair is suitable for qPCR analysis of GH gene expression in the test samples.
万方数据
维普
