This experiment focused on the design and evaluation of primers for growth hormone(GH)gene in Clarias gariepinus using fluorescence quantitative PCR(qPCR).Five pairs of primers were synthesized,and the GHF-GHR primer pair was selected based on the results of conventional PCR electrophoresis.Subsequently,the GHF-GHR primer pair was evaluated for qPCR.The results in-dicated that the amplification curve of the GH gene fragment with the GHF-GHR primer pair showed a single peak,demonstrating good specificity of the primers.The amplification efficiency of the GHF-GHR primer pair was determined to be 93.3%based on the standard curve,with an R2 value of 0.982,indicating a high amplification efficiency of the primers.These results suggest that the GHF-GHR primer pair is suitable for qPCR analysis of GH gene expression in the test samples.
维普
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