摘要
为筛选适应热带海水养殖环境并能高效净化养殖废水的优势藻种,分析比较五种微藻去除化学需氧量(COD)、总氮(TN)、总磷(TP)和亚硝酸盐氮(NO2--N)的能力.选择海南本土筛选的藻种小球藻(Chlo-rella sp.)、微拟球藻(Nannochloropsis sp.)、菱形藻(Nitzschia sp.)、牟氏角毛藻(Chaetoceros sp.)和外购藻种四尾栅藻(Scenedesmus sp.),在以对虾养殖水为底液配制的富营养化养殖废水中培养 6d.结果表明,绿藻在养殖废水中生长速率快,硅藻生长速率慢,小球藻在富营养化养殖废水中生长最好,OD680 值、叶绿素 a含量和细胞密度均最高,并与其他藻种有显著性差异.5 种藻种中COD去除效果好的藻种是菱形藻和小球藻,去除率分别为 71.6%、70.4%;总氮去除效果好的藻种是菱形藻、小球藻、牟氏角毛藻和微拟球藻,去除率分别为 50.36%、47.96%、46.41%、42.79%;总磷去除效果好的藻种是菱形藻,去除率为 71.43%;亚硝酸盐氮去除效果好的藻种是菱形藻、四尾栅藻和牟氏角毛藻,2d去除率分别为 96.60%、95.63%和 95.40%;综合所有指标,菱形藻对养殖废水综合净化效果最好.
Abstract
In order to screen the dominant algae that can adapt to the tropical marine aquaculture envi-ronment and purify aquaculture wastewater efficiently,the ability of five microalgae to remove chemi-cal oxygen demand(COD),total nitrogen(TN),total phosphorus(TP)and nitrite nitrogen(NO-2 N)was analyzed and compared.Chlorella sp.,Nannochloropsis sp.,Nitzschia sp.,Chaetoceros sp.,which were locally screened in Hainan,and purchased species Scenedesmus sp.were selected and cul-tured in eutrophic aquaculture wastewater with shrimp aquaculture water as bottom solution for 6 days.The results showed that Chlorophyta grew faster and diatom grew slower in aquaculture wastewater.Chlorella sp.grew best in eutrophic aquaculture wastewater,with the highest OD680 val-ue,chlorophyll-a content and cell density,which were significantly different from other algae species(P<0.05).Among the five kinds of algae,the ones with good COD removal effect were Nitzschia sp.and Chlorella sp.,and the removal rates were 71.6%and 70.4%respectively.The algae species with good removal efficiency of total nitrogen were Nitzschia sp.,Chlorella sp.,Chaetoceros sp.and Nannochloropsis sp.,and the removal rates were 50.36%,47.96%,46.41%and 42.79%respective-ly.Nitzschia sp.was the best algae to remove total phosphorus,and the removal rate was 71.43%.The algae species with good removal effect of nitrite nitrogen were Nitzschia sp.,Scenedesmus sp.and Chaetoceros sp.,The removal rates in two days were 96.60%,95.63%and 95.40%respectively.Nitzschia sp.had the best comprehensive purification effect on aquaculture wastewater.
基金项目
海南省自然科学基金(322RC766)
湛江市科技计划(2022A01050)
中国热带农科院基本科研业务费专项(1630102023007)
中国热带农科院公益项目(1630052019012)
维普
万方数据