The effects of chronic eugenol anesthesia on the blood physiology and the expression levels of hsp70 and hsp90 mRNA in the liver of red crucian carp
To investigate the effects of chronic anesthesia with eugenol on the blood physiology and ex-pression of stress genes hsp70 and hsp90 in the liver of red crucian carp(Carassius auratus),in exper-imental group,red crucian carps in an average body weight of(61.9±3.66)g were exposed to 15 mg/L eugenol for 12 h and fish without eugenol treatment was used as control group.The experimen-tal results showed that after 12 h anesthesia,no dead fish were observed and all fish exhibited normal behavior except for slower swimming.Compared with the control group,the blood cortisol content and lactate dehydrogenase activity of the fish subjected to eugenol treatment significantly decreased,while blood glucose content and K+,Na+concentrations significantly increased.The gene expression quantification results showed that the relative expression levels of hsp70 and hsp90 in the liver of fish in the eugenol treatment group were significantly higher than those in the control group.In summary,15 mg/L eugenol treatment did not cause significant damage to red crucian carp within 12 h and can be used for its live transportation.
red crucian carp(Carassius auratus)eugenolblood physiologyhsp70hsp90
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