Acute toxicity test of marine biocide to juvenile Lateolabrax japonicus
To investigate the acute toxicity of a marine biocide(active ingredient:biguanide salt poly-mer)to juvenile Lateolabrax japonicus,specimens with body length of(2.11±0.11)cm and body weight of(0.14±0.03)g were selected as experimental subjects and randomly divided into 7 groups,including 6 different concentration experimental groups and 1 blank control group.The ex-periments were conducted using a semi-static method,and the mortality and poisoning symptoms of the experimental fish were observed and recorded at 1,8,16,24,48,72,and 96 hours after the start of the experiment.The results showed that there was no mortality at the concentration of 10 mg/L of marine biocide,and mortality began to occur at 20 mg/L,and all experimental fish died at concentra-tions exceeding 40 mg/L;according to the experimental data,the 96-hour median lethal concentra-tion(96h-LC50)was approximately 20.05 mg/L,the 95%confidence lower limit was 15.61 mg/L,the confidence upper limit was 24.45 mg/L,and the safety concentration was 2.01 mg/L.
marine biocidebiguanide salt polymerjuvenileLateolabrax japonicusacute toxicity